The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase

被引：62
作者：
De Bie, LGS
Roovers, M
Oudjama, Y
Wattiez, R
Tricot, C
Stalon, V
Droogmans, L
Bujnicki, JM
机构：
[1] Free Univ Brussels, Microbiol Lab, Inst Rech Microbiol JM Wiame, B-1070 Brussels, Belgium
[2] Univ Mons, Chim Biol Lab, B-7000 Mons, Belgium
[3] Int Inst Mol & Cell Biol, Bioinformat Lab, PL-02109 Warsaw, Poland
来源：
JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 10期
关键词：
D O I：
10.1128/JB.185.10.3238-3243.2003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-L-methionine-dependent formation of N-7-methylguanosine at position 46 (m(7)G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m(7)G46) methyltransferase activity.
引用
收藏
页码：3238 / 3243
页数：6
相关论文
共 18 条
  • [1] Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA
    Alexandrov, A
    Martzen, MR
    Phizicky, EM
    [J]. RNA, 2002, 8 (10) : 1253 - 1266
  • [2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [3] Comparative genomics and evolution of proteins involved in RNA metabolism
    Anantharaman, V
    Koonin, EV
    Aravind, L
    [J]. NUCLEIC ACIDS RESEARCH, 2002, 30 (07) : 1427 - 1464
  • [4] 7-METHYLGUANINE SPECIFIC TRANSFER-RNA-METHYLTRANSFERASE FROM ESCHERICHIA-COLI
    ASCHHOFF, HJ
    ELTEN, H
    ARNOLD, HH
    MAHAL, G
    KERSTEN, W
    KERSTEN, H
    [J]. NUCLEIC ACIDS RESEARCH, 1976, 3 (11) : 3109 - 3122
  • [5] Bjork Glenn R., 1995, P165
  • [6] Bujnicki Janusz M., 2001, Acta Microbiologica Polonica, V50, P7
  • [7] mRNA: guanine-N7 cap methyltransferases: identification of novel members of the family, evolutionary analysis, homology modeling, and analysis of sequence-structure-function relationships
    Bujnicki, Janusz M.
    Feder, Marcin
    Radlinska, Monika
    Rychlewski, Leszek
    [J]. BMC BIOINFORMATICS, 2001, 2 (1)
  • [8] CHROMOSOMAL LOCATION AND CLONING OF THE GENE (TRMD) RESPONSIBLE FOR THE SYNTHESIS OF TRANSFER RNA(M1G)METHYLTRANSFERASE IN ESCHERICHIA-COLI K-12
    BYSTROM, AS
    BJORK, GR
    [J]. MOLECULAR & GENERAL GENETICS, 1982, 188 (03): : 440 - 446
  • [9] NUCLEOTIDE SEQUENCE OF METHIONINE TRANSFER RNAM
    CORY, S
    MARCKER, KA
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1970, 12 (01): : 177 - &
  • [10] Fauman EB., 1999, S ADENOSYLMETHIONINE, P1, DOI DOI 10.1142/9789812813077_0001
12