Characterization of a nontoxic pyomelanin pigment produced by the yeast Yarrowia lipolytica

被引：45

作者：

Ben Tahar, Imen ^{[1
]}

Kus-Liskiewicz, Malgorzata ^{[2
]}

Lara, Yannick ^{[3
]}

Javaux, Emmanuelle ^{[3
]}

Fickers, Patrick ^{[1
]}

机构：

[1] Univ Liege, Gembloux Agro Bio Tech, TERRA Teaching & Res Ctr, Microbial Proc & Interact, Gembloux, Belgium

[2] Univ Rzeszow, Fac Biotechnol, Rzeszow, Poland

[3] Univ Liege, Geol Dept, UR Astrobiol, Early Life Traces & Evolut Astrobiol, Gembloux, Belgium

来源：

BIOTECHNOLOGY PROGRESS | 2020年 / 36卷 / 02期

关键词：

HGA-melanin pathway; noncytotoxic; photoprotection; pyomelanin; Yarrowia lipolytica; COLLECTIVE CELL-MIGRATION; MELANIN SYNTHESIS; STRATEGY; STRAIN;

D O I：

10.1002/btpr.2912

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, we report on the ability of the yeast Yarrowia lipolytica W29 to produce an extracellular melanin-like brown pigment at high yield (0.5 mg/ml) in culture medium supplemented with L-tyrosine. This pigment has been characterized as pyomelanin and its synthesis was found to occur by the so-called HGA-melanin pathway. The purified pyomelanin was found embedded with antioxidant properties as it exhibited a radical scavenging activity toward 1,1-diphenyl-2-picrylhydrazyl (DPPH) with IC50 of 230 mu g/ml. It was also characterized as noncytotoxic toward two mammalian cell lines, namely the mouse fibroblast NIH3T3 and human keratinocytes HaCaT. When blended with different commercial sunscreens, the purified pyomelanin increased significantly the sun protection factor (SPF) value, highlighting its potential utilization as UV-filter in cosmetic preparations.

引用

页数：10