Genetic tool development and systemic regulation in biosynthetic technology

被引：14

作者：

Dai, Zhongxue ^{[1
]}

Zhang, Shangjie ^{[1
]}

Yang, Qiao ^{[1
]}

Zhang, Wenming ^{[1
,2
]}

Qian, Xiujuan ^{[1
]}

Dong, Weiliang ^{[1
,2
]}

Jiang, Min ^{[1
,2
]}

Xin, Fengxue ^{[1
,2
]}

机构：

[1] Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Puzhu South Rd 30, Nanjing 211800, Jiangsu, Peoples R China

[2] Nanjing Tech Univ, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing 211800, Jiangsu, Peoples R China

来源：

BIOTECHNOLOGY FOR BIOFUELS | 2018年 / 11卷

基金：

中国国家自然科学基金;

关键词：

Synthetic biology; Genetic engineering; Synthetic genomics; Genetic tools; CHEMICAL-SYNTHESIS; SYNTHETIC BIOLOGY; EFFICIENT PRODUCTION; METABOLIC PATHWAY; ESCHERICHIA-COLI; TOGGLE SWITCH; GENOME; EXPRESSION; DESIGN; YEAST;

D O I：

10.1186/s13068-018-1153-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

With the increased development in research, innovation, and policy interest in recent years, biosynthetic technology has developed rapidly, which combines engineering, electronics, computer science, mathematics, and other disciplines based on classical genetic engineering and metabolic engineering. It gives a wider perspective and a deeper level to perceive the nature of life via cell mechanism, regulatory networks, or biological evolution. Currently, synthetic biology has made great breakthrough in energy, chemical industry, and medicine industries, particularly in the programmable genetic control at multiple levels of regulation to perform designed goals. In this review, the most advanced and comprehensive developments achieved in biosynthetic technology were represented, including genetic engineering as well as synthetic genomics. In addition, the superiority together with the limitations of the current genome-editing tools were summarized.

引用

页数：12

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