A low detection limit penicillin biosensor based on single graphene nanosheets preadsorbed with hematein/ionic liquids/penicillinase

被引：37
作者：
Wu, Yueting [1 ,2 ,3 ]
Tang, Lele [1 ,2 ,3 ]
Huang, Linhong [1 ,2 ,3 ]
Han, Zhizhong [2 ,3 ]
Wang, Jian [3 ]
Pan, Haibo [1 ,2 ,3 ]
机构：
[1] Fuzhou Univ, Fujian Key Lab Med Instrument & Pharmaceut Techno, Fuzhou 350002, Fujian, Peoples R China
[2] Fuzhou Univ, Inst Res Funct Mat, Fuzhou 350002, Fujian, Peoples R China
[3] Fuzhou Univ, Coll Chem & Chem Engn, Fuzhou 350108, Fujian, Peoples R China
来源：
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2014年 / 39卷
关键词：
Penicillin biosensor; Electrochemistry; Graphene nanosheets; Ionic liquids; Hematein; IONIC LIQUID; FUNCTIONALIZED GRAPHENE; DIRECT ELECTROCHEMISTRY; CARBON NANOTUBES; ELECTRODE; OXIDE; MILK; ELECTROCATALYSIS; DISPERSIONS; ANTIBODIES;
D O I：
10.1016/j.msec.2014.02.012
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
In this study, we reported on a low detection limit penicillin biosensor with layer-by-layer (LbL) film containing single-graphene nanosheets (SGNs) preadsorbed with hematein, ionic liquids (ILs) and penicillinase. The penicillinase catalyzes the hydrolysis of penicillin to penicilloic acid, where H+ is liberated and monitored amperometrically with hematein as a pH indicator. The SGN-hemateinas/penicillinase biosensor exhibited excellent performance for penicillin in PBS with a wide range from 1.25 x 10(-13) to 7.5 x 10(-3) M, and a low detection limit of 10(-13) M (0.04 ppt, S/N >= 3). Furthermore, the detection of penicillin concentration in real sample (milk) had acceptable accuracy with the assay system. (C) 2014 Elsevier B.V. All rights reserved.
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收藏
页码：92 / 99
页数：8
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