Characterization and antioxidant activity of semi-purified extracts and pure delphinidin-glycosides from eggplant peel (Solanum melongena L.)

被引：34

作者：

Braga, Pier Carlo ^{[1
]}

Lo Scalzo, Roberto ^{[2
]}

Dal Sasso, Monica ^{[1
]}

Lattuada, Norma ^{[2
]}

Greco, Valentina ^{[1
]}

Fibiani, Marta ^{[2
]}

机构：

[1] Univ Milan, Sch Med, Dept Pharmacol, Via Vanvitelli 32, I-20129 Milan, Italy

[2] Consiglio Ric Agr & Anal Econ Agr, CREA IAA Unita Ric Proc Ind Agroalimentare, Via Venezian 26, I-20133 Milan, Italy

来源：

JOURNAL OF FUNCTIONAL FOODS | 2016年 / 20卷

关键词：

Delphinidin-3-rutinoside; Nasunin; Antioxidant compounds; Electron paramagnetic resonance; Human neutrophils oxidative burst; ACYLATED ANTHOCYANINS; NASUNIN; COLOR;

D O I：

10.1016/j.jff.2015.10.032

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Eggplant peels containing delphinidin-3-rutinoside or delphinidin-3-(p-coumaroylrutinoside)-5-glucoside (nasunin) were compared and characterized for antioxidant properties, using three cell-free assays with electron paramagnetic resonance (EPR) and two biological assays on human neutrophils with luminol amplified chemiluminescence (LACL). Antioxidant solutions were made with semi-purified extracts depleted in chlorogenic acid or with purified anthocyanin crystals. The semi-purified nasunin extract was higher in scavenging capacity than delphinidin-3-rutinoside in four assays (superoxide anion, hydroxyl radical, LACL, and Fremy's salt). Nasunin crystals showed higher antioxidant indices in Fremy's salt and LACL assays, with no detectable activity against the hydroxyl radical. The EC50 against free radicals was 0.4-20.6 nmol/mL in semi-purified extracts, and 1.7-73.6 nmol/mL for anthocyanin crystals. The higher activity of semi-purified extracts than crystals was attributed to other antioxidants, such as ascorbic acid, glutathione and biogenic amines, thus representing a percentage close to 30% of the total antioxidant content of eggplant peels. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：411 / 421

页数：11

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