Efficient proximity labeling in living cells and organisms with TurboID

被引：1016

作者：

Branon, Tess C. ^{[1
,2
,3
,4
]}

Bosch, Justin A. ^{[5
]}

Sanchez, Ariana D. ^{[4
]}

Udeshi, Namrata D. ^{[6
]}

Svinkina, Tanya ^{[6
]}

Carr, Steven A. ^{[6
]}

Feldman, Jessica L. ^{[4
]}

Perrimon, Norbert ^{[5
,7
]}

Ting, Alice Y. ^{[1
,2
,3
,4
,8
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

[2] Stanford Univ, Dept Genet, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[4] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

[5] Harvard Med Sch, Dept Genet, Boston, MA USA

[6] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA

[7] Howard Hughes Med Inst, Boston, MA 02115 USA

[8] Chan Zuckerberg Biohub, San Francisco, CA 94158 USA

来源：

NATURE BIOTECHNOLOGY | 2018年 / 36卷 / 09期

关键词：

CAENORHABDITIS-ELEGANS; BIOTIN REPRESSOR; PROTEIN; PEROXIDASE; IDENTIFICATION; COMPONENTS; REPORTER; TOPOLOGY; KINASE; LIGASE;

D O I：

10.1038/nbt.4201

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Protein interaction networks and protein compartmentalization underlie all signaling and regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a new approach to study the spatial and interaction characteristics of proteins in living cells. However, current PL methods require over 18 h of labeling time or utilize chemicals with limited cell permeability or high toxicity. We used yeast display-based directed evolution to engineer two promiscuous mutants of biotin ligase, TurboID and miniTurbo, which catalyze PL with much greater efficiency than BioID or BioID2, and enable 10-min PL in cells with non-toxic and easily deliverable biotin. Furthermore, TurboID extends biotin-based PL to flies and worms.

引用

页码：880 / +

页数：19

共 50 条

[1] Efficient proximity labeling in living cells and organisms with TurboID
Tess C Branon
Justin A Bosch
Ariana D Sanchez
Namrata D Udeshi
Tanya Svinkina
Steven A Carr
Jessica L Feldman
Norbert Perrimon
Alice Y Ting
Nature Biotechnology, 2018, 36 : 880 - 887
[2] Author Correction: Efficient proximity labeling in living cells and organisms with TurboID
Tess C. Branon
Justin A. Bosch
Ariana D. Sanchez
Namrata D. Udeshi
Tanya Svinkina
Steven A. Carr
Jessica L. Feldman
Norbert Perrimon
Alice Y. Ting
Nature Biotechnology, 2020, 38 : 108 - 108
[3] Efficient proximity labeling in living cells and organisms with TurboID (vol 47, pg 156, 2019
Branon, Tess C.
Bosch, Justin A.
Sanchez, Ariana D.
Udeshi, Namrata D.
Svinkina, Tanya
Carr, Steven A.
Feldman, Jessica L.
Perrimon, Norbert
Ting, Alice Y.
NATURE BIOTECHNOLOGY, 2020, 38 (01) : 108 - 108
[4] Proximity labeling in mammalian cells with TurboID and split-TurboID
Cho, Kelvin F.
Branon, Tess C.
Udeshi, Namrata D.
Myers, Samuel A.
Carr, Steven A.
Ting, Alice Y.
NATURE PROTOCOLS, 2020, 15 (12) : 3971 - 3999
[5] Proximity labeling in mammalian cells with TurboID and split-TurboID
Kelvin F. Cho
Tess C. Branon
Namrata D. Udeshi
Samuel A. Myers
Steven A. Carr
Alice Y. Ting
Nature Protocols, 2020, 15 : 3971 - 3999
[6] Proximity labeling with TurboID
Allison Doerr
Nature Methods, 2018, 15 : 764 - 764
[7] Proximity labeling with TurboID
Doerr, Allison
NATURE METHODS, 2018, 15 (10) : 764 - 764
[8] Highly efficient identification of nucleocytoplasmic O-glycosylation by the TurboID-based proximity labeling method in living cells
Long, Yunfeng
Li, Zhunjie
Wang, Long
Ao, Xin
Zhang, Zhengrong
Chen, Qingjie
Zhu, Dan
Liu, Xinghui
Liu, Ruolan
Chen, Banghang
Zhu, He
Su, Yanting
BIOTECHNOLOGY JOURNAL, 2024, 19 (01)
[9] Split-TurboID enables contact-dependent proximity labeling in cells
Cho, Kelvin F.
Branon, Tess C.
Rajeev, Sanjana
Svinkina, Tanya
Udeshi, Namrata D.
Thoudam, Themis
Kwak, Chulhwan
Rhee, Hyun-Woo
Lee, In-Kyu
Carr, Steven A.
Ting, Alice Y.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (22) : 12143 - 12154
[10] Efficient TurboID-based proximity labelling method for identifying terminal sialic acid glycosylation in living cells
Liu, Wu
Long, Yunfeng
Bao, Yongfen
Li, Yang
Deng, Mengxue
Yang, Xiuyi
Zhu, He
Su, Yanting
ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2022, 54 (12) : 1841 - 1853

← 1 2 3 4 5 →