Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart

被引：26
作者：
Kolarova, J. [1 ]
Fialova, K. [2 ]
Janousek, O. [1 ]
Novakova, M. [2 ]
Provaznik, I. [1 ]
机构：
[1] Brno Univ Technol, Dept Biomed Engn, Kolejni 4, Brno 61200, Czech Republic
[2] Masaryk Univ, Fac Med, Dept Physiol, Brno, Czech Republic
来源：
PHYSIOLOGICAL RESEARCH | 2010年 / 59卷
关键词：
Isolated heart; Action potential; Electrogram; Voltage sensitive dye; Ratiometry; FLUORESCENT DYE EMISSION; SPATIAL HETEROGENEITY; MOTION ARTIFACT; ISCHEMIA; WAVELENGTH; CALCIUM; PROBES;
D O I：
10.33549/physiolres.932010
中图分类号：
Q4 [生理学];
学科分类号：
071003 ;
摘要：
Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.
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收藏
页码：S71 / S80
页数：10
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