Guide-independent DNA cleavage by archaeal Argonaute from Methanocaldococcus jannaschii

被引：92

作者：

Zander, Adrian ^{[1
,2
]}

Willkomm, Sarah ^{[1
,2
]}

Ofer, Sapir ^{[3
]}

van Wolferen, Marleen ^{[4
]}

Egert, Luisa ^{[1
,2
]}

Buchmeier, Sabine ^{[5
]}

Stoeckl, Sarah ^{[1
,2
]}

Tinnefeld, Philip ^{[5
]}

Schneider, Sabine ^{[6
]}

Klingl, Andreas ^{[7
]}

Albers, Sonja-Verena ^{[4
]}

Werner, Finn ^{[3
]}

Grohmann, Dina ^{[1
,2
]}

机构：

[1] Univ Regensburg, Dept Microbiol, D-93053 Regensburg, Germany

[2] Univ Regensburg, Archaea Ctr, D-93053 Regensburg, Germany

[3] UCL, Inst Struct & Mol Biol, Div Biosci, London WC1E 6BT, England

[4] Univ Freiburg, Inst Biol 2, Mol Biol Archaea, Microbiol, Schaenzlestr 1, D-79104 Freiburg, Germany

[5] Tech Univ Braunschweig BRICS, Inst Phys & Theoret Chem NanoBioSci, Rebenring 56, D-38106 Braunschweig, Germany

[6] Tech Univ Munich, Dept Chem, CIPSM, Lichtenbergstr 4, D-85748 Garching, Germany

[7] Bioctr LMU Munich, Dept Biol Plant Dev 1, Grosshadernerstr 2-4, D-82152 Planegg Martinstried, Germany

来源：

NATURE MICROBIOLOGY | 2017年 / 2卷 / 06期

基金：

欧洲研究理事会;

关键词：

STRUCTURAL BASIS; BACTERIAL ARGONAUTE; SILENCING COMPLEX; RNA INTERFERENCE; RECOGNITION; SULFOLOBUS; PROTEINS; GENUS;

D O I：

10.1038/nmicrobiol.2017.34

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Prokaryotic Argonaute proteins acquire guide strands derived from invading or mobile genetic elements, via an unknown pathway, to direct guide-dependent cleavage of foreign DNA. Here, we report that Argonaute from the archaeal organism Methanocaldococcus jannaschii (MjAgo) possesses two modes of action: the canonical guide-dependent endonuclease activity and a non-guided DNA endonuclease activity. The latter allows MjAgo to process long double-stranded DNAs, including circular plasmid DNAs and genomic DNAs. Degradation of substrates in a guide-independent fashion primes MjAgo for subsequent rounds of DNA cleavage. Chromatinized genomic DNA is resistant to MjAgo degradation, and recombinant histones protect DNA from cleavage in vitro. Mutational analysis shows that key residues important for guide-dependent target processing are also involved in guide-independent MjAgo function. This is the first characterization of guide-independent cleavage activity for an Argonaute protein potentially serving as a guide biogenesis pathway in a prokaryotic system.

引用

页数：10

共 35 条

[1] Inducible and constitutive promoters for genetic systems in Sulfolobus acidocaldarius [J].

Berkner, Silvia ;

Wlodkowski, Alexander ;

Albers, Sonja-Verena ;

Lipps, Georg .

EXTREMOPHILES, 2010, 14 (03) :249-259

[2] The Epigenomic Landscape of Prokaryotes [J].

Blow, Matthew J. ;

Clark, Tyson A. ;

Daum, Chris G. ;

Deutschbauer, Adam M. ;

Fomenkov, Alexey ;

Fries, Roxanne ;

Froula, Jeff ;

Kang, Dongwan D. ;

Malmstrom, Rex R. ;

Morgan, Richard D. ;

Posfai, Janos ;

Singh, Kanwar ;

Visel, Axel ;

Wetmore, Kelly ;

Zhao, Zhiying ;

Rubin, Edward M. ;

Korlach, Jonas ;

Pennacchio, Len A. ;

Roberts, Richard J. .

PLOS GENETICS, 2016, 12 (02)

[3] SULFOLOBUS - NEW GENUS OF SULFUR-OXIDIZING BACTERIA LIVING AT LOW PH AND HIGH-TEMPERATURE [J].

BROCK, TD ;

BROCK, KM ;

BELLY, RT ;

WEISS, RL .

ARCHIV FUR MIKROBIOLOGIE, 1972, 84 (01) :54-&

[4] A Dynamic Search Process Underlies MicroRNA Targeting [J].

Chandradoss, Stanley D. ;

Schirle, Nicole T. ;

Szczepaniak, Malwina ;

MacRae, Ian J. ;

Joo, Chirlmin .

CELL, 2015, 162 (01) :96-107

[5] A Dicer-independent miRNA biogenesis pathway that requires Ago catalysis [J].

Cheloufi, Sihem ;

Dos Santos, Camila O. ;

Chong, Mark M. W. ;

Hannon, Gregory J. .

NATURE, 2010, 465 (7298) :584-U76

[6] RNA interference is mediated by 21-and 22-nucleotide RNAs [J].

Elbashir, SM ;

Lendeckel, W ;

Tuschl, T .

GENES & DEVELOPMENT, 2001, 15 (02) :188-200

[7] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].

Elbashir, SM ;

Harborth, J ;

Lendeckel, W ;

Yalcin, A ;

Weber, K ;

Tuschl, T .

NATURE, 2001, 411 (6836) :494-498

[8]

Elkayam E, 2012, CELL, V150, P100, DOI 10.1016/j.cell.2012.05.017

[9] Structural basis for 5′-nucleotide base-specific recognition of guide RNA by human AGO2 [J].

Frank, Filipp ;

Sonenberg, Nahum ;

Nagar, Bhushan .

NATURE, 2010, 465 (7299) :818-822

[10]

Gao F, 2016, NAT BIOTECHNOL, V34, P768, DOI 10.1038/nbt.3547

← 1 2 3 4 →