A link between rnRNA turnover and RNA interference in Arabidopsis

被引：251

作者：

Gazzani, S

Lawrenson, T

Woodward, C

Headon, D

Sablowski, R ^{[1
]}

机构：

[1] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

[2] Univ Washington, Dept Biol, Seattle, WA 98195 USA

[3] Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England

来源：

SCIENCE | 2004年 / 306卷 / 5698期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1126/science.1101092

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In RNA interference (RNAi), double-stranded RNA (dsRNA) triggers degradation of homologous messenger RNA. In many organisms, RNA-dependent RNA polymerase (RdRp) is required to initiate or amplify RNAi, but the substrate for dsRNA synthesis in vivo is not known. Here, we show that RdRp-dependent transgene silencing in Arabidopsis was caused by mutation of XRN4, which is a ribonuclease (RNase) implicated in mRNA turnover by means of decapping and 5'-3' exonucteotysis. When both XRN4 and the RdRp were mutated, the plants accumulated decapped transgene mRNA. We propose that mRNAs lacking a cap structure become exposed to RdRp to initiate or maintain RNAi.

引用

页码：1046 / 1048

页数：3

共 29 条

[1] The 3′ to 5′ degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3′ to 5′ exonucleases of the exosome complex [J].