LncRNA FOXD2-AS1 Regulates miR-25-3p/Sema4c Axis To Promote The Invasion And Migration Of Colorectal Cancer Cells

被引：31
作者：
Zhang, Mengyan [1 ]
Jiang, Xiang [2 ]
Jiang, Sumei [3 ]
Guo, Zhongying [4 ]
Zhou, Qinfeng [5 ]
He, Jingdong [1 ]
机构：
[1] Nanjing Med Univ, Dept Oncol, Affiliated Huaian 1 Peoples Hosp, 6 Beijing West Rd, Huaian City 223300, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Courage Pancreas Surg, Affiliated Huaian 1 Peoples Hosp, Huaian City 223300, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Ultrason Dept, Affiliated Huaian 1 Peoples Hosp, Huaian City 223300, Jiangsu, Peoples R China
[4] Nanjing Med Univ, Dept Pathol, Affiliated Huaian 1 Peoples Hosp, Huaian City 223300, Jiangsu, Peoples R China
[5] Nanjing Univ Chinese Med, Dept Lab Med, Zhangjiagang TCM Hosp, Nanjing 215600, Jiangsu, Peoples R China
来源：
CANCER MANAGEMENT AND RESEARCH | 2019年 / 11卷
关键词：
colorectal cancer; FOXD2-AS1; miR-25-3p; Sema4C; survival; PROLIFERATION;
D O I：
10.2147/CMAR.S228628
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Purpose: Although the roles of lncRNA FOXD2-AS1 have been investigated in many types of cancers including colorectal cancer (CRC), its functionality remains to be further investigated. Analysis of the TCGA data set revealed that FOXD2-AS1 was up-regulated in CRC tissues. This study aimed to analyze the function of FOXD2-AS1 in CRC. Methods: FOXD2-AS1 expression was detected by qPCR. A 5-year follow-up study was performed to analyze the prognostic value of FOXD2-AS1 for CRC. Overexpression experiments were performed to analyze the interactions among FOXD2-AS1, miR-25-3p and Sema4C. Transwell assays were performed to analyze cell invasion and migration. Results: In this study, we further confirmed the up-regulation of FOXD2-AS1 in CRC patients and showed that high FOXD2-AS1 level predicted poor survival. Bioinformatics analysis showed that miR-25-3p may bind FOXD2-AS1, while over-expression experiments showed no effects on each other's expression. Instead, FOXD2-AS1 over-expression led to the up-regulate Sema4C, which is a target of miR-25-3p. Transwell assay showed that FOXD2-AS1 and Sema4C over-expression led to the increased invasion and migration rates of CRC cells. MiR-25-3p plays the opposite role and attenuated the effects of FOXD2-AS1 and Sema4C over-expression. Conclusion: FOXD2-AS1 may regulate the miR-25-3p/Sema4C axis to promote the invasion and migration of CRC cells.
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收藏
页码：10633 / 10639
页数：7
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