Effects of exposure to BDE-99 on oxidative status of liver and kidney in adult rats

被引:79
作者
Albina, Maria L. [1 ,2 ]
Alonso, Virginia [1 ,2 ]
Linares, Victoria [1 ,2 ]
Belles, Montserrat [1 ,2 ]
Sirvent, Juan J. [3 ]
Domingo, Jose L. [2 ]
Sanchez, Domenec J. [1 ,2 ]
机构
[1] Univ Rovira & Virgili, Sch Med, Physiol Unit, IISPV, E-43201 Reus, Catalonia, Spain
[2] Univ Rovira & Virgili, Sch Med, IISPV, Lab Toxicol & Environm Hlth, E-43201 Reus, Catalonia, Spain
[3] Univ Rovira & Virgili, Sch Med, Pathol Unit, IISPV, E-43201 Reus, Catalonia, Spain
关键词
BDE-99; Adult rats; Oxidative stress; Hepatotoxicity; Nephrotoxicity; POLYBROMINATED DIPHENYL ETHERS; BROMINATED FLAME RETARDANTS; INDUCED NEPHROTOXICITY; ANTIOXIDANT STATUS; ENZYME-ACTIVITIES; TISSUE; GLUTATHIONE; METABOLISM; STRESS; PBDES;
D O I
10.1016/j.tox.2010.03.006
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Little is known about the potential toxicity of polybrominated diphenyl ethers (PBDEs) on hepatic and renal tissues. In this study, we investigated the modifications in endogenous antioxidant capacity and oxidative damage in liver and kidney of rats by exposure to one of the most persistent PBDE congeners. the 2,2',4,4',5-pentabromodiphenyl ether (BDE-99). Adult male rats (10 per group) received BDE-99 by gavage at a single dose of 0, 0.6, and 1.2mg/kg body weight. Forty-five days after exposure, liver and kidney were removed and processed to examine the following oxidative stress (OS) markers: reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione reductase (CR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), and thiobarbituric acid reactive substances (TBARS). In liver, BDE-99 significantly increased SOD activity, GSSG levels, and GSSG/GSH ratio, while GSH levels decreased. Moreover, CAT activity was only reduced at the highest BDE-99 dose. In kidney, CAT activity was significantly decreased, while GSSG/GSH ratio significantly increased following BDE-99 exposure at 1.2mg/kg body weight. Histological examination of tissues showed phagolysosomes in the kidneys of BDE-99-exposed rats. The results of this investigation suggest that acute oral BDE-99 exposure causes renal and liver impairment, being oxidative damage a potential mechanism for nephrotoxicity and hepatotoxicity. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:51 / 56
页数:6
相关论文
共 50 条
[1]  
[Anonymous], ENV HLTH CRIT
[2]   Naringenin attenuates cisplatin nephrotoxicity in rats [J].
Badary, OA ;
Abdel-Maksoud, S ;
Ahmed, WA ;
Owieda, GH .
LIFE SCIENCES, 2005, 76 (18) :2125-2135
[3]   Dietary intake and risk evaluation of polybrominated diphenyl ethers in The Netherlands [J].
Bakker, Martine I. ;
de Winter-Sorkina, Renata ;
de Mul, Anika ;
Boon, Polly E. ;
van Donkersgoed, Gerda ;
van Klaveren, Jacob D. ;
Baumann, Bert A. ;
Hijman, Willie C. ;
van Leeuwen, Stefan P. J. ;
de Boer, Jacob ;
Zeilmaker, Marco J. .
MOLECULAR NUTRITION & FOOD RESEARCH, 2008, 52 (02) :204-216
[4]   Role of α-tocopherol on antioxidant status in liver, lung and kidney of PCB exposed male albino rats [J].
Banudevi, Sivanantham ;
Krishnamoorthy, Gunasekaran ;
Venkataraman, Prabhu ;
Vignesh, Chandraganth ;
Aruldhas, Maria Michael ;
Arunakaran, Jagadeesan .
FOOD AND CHEMICAL TOXICOLOGY, 2006, 44 (12) :2040-2046
[5]   Melatonin reduces uranium-induced nephrotoxicity in rats [J].
Belles, Montserrat ;
Linares, Victoria ;
Albina, M. Luisa ;
Sirvent, Joan ;
Sanchez, Domenec J. ;
Domingo, Jose L. .
JOURNAL OF PINEAL RESEARCH, 2007, 43 (01) :87-95
[6]   Brominated flame retardants: Cause for concern? [J].
Birnbaum, LS ;
Staskal, DF .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2004, 112 (01) :9-17
[7]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]   Novel evidence suggesting an anti-oxidant property for erythropoietin on vancomycin-induced nephrotoxicity in a rat model [J].
Cetin, Hasan ;
Olgar, Seref ;
Oktem, Faruk ;
Ciris, Metin ;
Uz, Efkan ;
Aslan, Cagatay ;
Ozguner, Fehmi .
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2007, 34 (11) :1181-1185
[9]   Metabolism and disposition of 2,2′,4,4′,5-pentabromodiphenyl ether (BDE99) following a single or repeated administration to rats or mice [J].
Chen, L. -J. ;
Lebetkin, E. H. ;
Sanders, J. M. ;
Burka, L. T. .
XENOBIOTICA, 2006, 36 (06) :515-534
[10]  
Chirino Yolanda I., 2004, BMC Pharmacology, V4, P20, DOI 10.1186/1471-2210-4-20