NMR methods for studying membrane-active antimicrobial peptides

被引：131
作者：
Strandberg, E
Ulrich, AS [1 ]
机构：
[1] Forschungszentrum Karlsruhe, IFIA, D-76012 Karlsruhe, Germany
[2] Univ Karlsruhe, Inst Organ Chem, D-76131 Karlsruhe, Germany
来源：
CONCEPTS IN MAGNETIC RESONANCE PART A | 2004年 / 23A卷 / 02期
关键词：
amphiphilic antimicrobial peptides; solid state NMR; biomembranes; lipid bilayers; lipid-peptide interactions; peptide structure determination; isotope labeling; H-1-; (2) H-; C-13_; (15) N-; F-19-; and P-31-NMR;
D O I：
10.1002/cmr.a.20024
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
NMR is a versatile tool for studying interactions between antimicrobial peptides and lipid membranes. Different approaches using both liquid state and solid state NMR are outlined here, with an emphasis on solid state NMR methods, to study the structures of antimicrobial peptides in lipid bilayers as well as the effect of these peptides on model membranes. Different NMR techniques for observing both peptides and lipids are explained, including H-2, C-13, N-15, and F-19 labels, or natural abundance H-1, C-13, or P-31. Previous studies in the field are extensively reviewed in easily accessible tables. (C) 2004 Wiley Periodicals, Inc.
引用
收藏
页码：89 / 120
页数：32
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