Changes in amount of ADM mRNA and RAMP2 mRNA in calcified vascular smooth muscle cells

被引:16
作者
Qi, YF
Wang, SH
Zhang, BH
Bu, DF
Shu, TC [1 ]
Du, JB
机构
[1] Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100034, Peoples R China
[2] Peking Univ, Ctr Hlth Sci, Dept Physiol, Beijing 100083, Peoples R China
[3] Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
关键词
vascular smooth muscle cells (VSMCs); adrenomedullin (ADM); receptor activity modifying protein 2 (RAMP2); mRNA;
D O I
10.1016/S0196-9781(03)00036-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This work was aimed to explore the changes and significance of adrenomedullin (ADM) mRNA and receptor activity modifying protein 2 (RAMP2) mRNA in calcified vascular smooth muscle cells (VSMCs). Calcification of cultured rat VSMCs was produced by incubation with beta-glycerophosphate. Content of ADM released by VSMCs was measured by radioimmunoassay (RIA). The amount of ADM mRNA and RAMP2 mRNA was determined by competitive quantitative RT-PCR. The intracellular calcium content, alkaline phosphatases activity and cellular Ca-45(2+)-uptake were determined. The results showed that the content of calcium, Ca-45(2+)-uptake and alkaline phosphatases activity in calcified VSMCs were increased by 118%, 174% and seven-fold (all P < 0.01), respectively, compared with control VSMCs. Content of ADM in medium was increased by 99% (P < 0.01). Furthermore, it was found that the amount of ADM mRNA and RAMP2 mRNA in calcified cells was elevated by 78 and 56% (all P < 0.05), respectively, compared with control. The elevated levels of RAMP2 mRNA were in positive correlation with ADM mRNA (r = 0.76, P < 0.05) in calcified VSMCs. In conclusion, calcified VSMCs generated an increased amount of ADM, and up-regulated gene expressions of ADM and RAMP2. (C) 2003 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:287 / 294
页数:8
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