High level secretion of recombinant staphylokinase into periplasm of Escherichia coli

被引：20
|
作者：
Lee, SJ
Kim, IC
Kim, DM
Bae, KH
Byun, SM
机构：
[1] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea
[2] Res Ctr New Biomat Agr, Taejon 305701, South Korea
来源：
BIOTECHNOLOGY LETTERS | 1998年 / 20卷 / 02期
关键词：
D O I：
10.1023/A:1005359920522
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The staphylokinase (SAK) gene from Staphylococcus aureus NCTC10033 was inserted into an expression vector, pKK-ompA, having a tac promoter and an ompA signal sequence. Escherichia coli JM109 carrying the recombinant plasmid produced and secreted the recombinant SAK (rSAK) at 15ug/ml into periplasm and 5ug/ml to extracellular media, respectively. The rSAK was purified with 59% yield by simple procedures from the periplasm of E. coli. The aminoterminal sequence and human plasminogen activating activity of rSAK were coincided with the authentic SAK.
引用
收藏
页码：113 / 116
页数：4
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