Spatial distribution and r-DNA second internal transcribed spacer characterization of Anopheles dirus (Diptera: Culicidae) complex species in north-east India

被引：11

作者：

Prakash, Anil ^{[1
]}

Sarma, D. K. ^{[1
]}

Bhattacharyya, D. R. ^{[1
]}

Mohapatra, P. K. ^{[1
]}

Bhattacharjee, K. ^{[1
]}

Das, K. ^{[1
]}

Mahanta, J. ^{[1
]}

机构：

[1] NE Indian Council Med Res, Reg Med Res Ctr, Dibrugarh 786001, Assam, India

来源：

ACTA TROPICA | 2010年 / 114卷 / 01期

关键词：

An; baimaii; ASPCR; Dirus complex; r-DNA ITS2; Malaria vector; Sibling species; Species X of China; RIBOSOMAL DNA; POPULATION-STRUCTURE; IDENTIFICATION; MOSQUITOS;

D O I：

10.1016/j.actatropica.2010.01.003

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The identity and distribution of the prevalent member species of the Anopheles dirus complex mosquitoes in the north-eastern region of India was investigated in a cross-sectional study. We altogether collected 267 individuals of An. dirus s.l. from 27 forested/forest fringed locations spread across the seven northeastern states, identified the species using a ribosomal DNA (r-DNA) second internal transcribed spacer (ITS2) based allele specific polymerase chain reaction (ASPCR) method and sequenced the ITS2 locus in a sub set of mosquitoes. An. baimaii was identified as the main, almost exclusive (266/267), species of the Dirus complex throughout the north-east India with no intraspecific variation in the 479 base pair long ITS2 sequences in 59 of the 60 individuals sequenced. Ribosomal DNA of one individual from Assam state did not amplify in the ASPCR, possessed 786 base pair long ITS2 sequence and showed 99.7% similarity with the sequence of An. dims species D (An. baimaii) from Yunnan province of China, later referred to as species X of the Dirus complex. These observations suggest the presence of another, possibly the new, species of the Dirus complex, sympatric with An. baimaii, in Assam warranting investigations on its distribution, biology and role in human malaria transmission in north-east India. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：49 / 54

页数：6

共 26 条

[1]

BAIMAI V, 1988, Southeast Asian Journal of Tropical Medicine and Public Health, V19, P151

[2]

BAIMAI V, 1989, P 3 C MAL RES THAIL, P146

[3] DISCRIMINATION OF ALL MEMBERS OF THE ANOPHELESPUNCTULATUS COMPLEX BY POLYMERASE CHAIN-REACTION RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS [J].

BEEBE, NW ;

SAUL, A .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1995, 53 (05) :478-481

[4]

BHAT H R, 1988, Indian Journal of Malariology, V25, P103

[5]

Das Bina Pani, 1990, Zoology (Journal of Pure and Applied Zoology), V2, P131

[6] CONCERTED EVOLUTION OF REPETITIVE DNA-SEQUENCES IN EUKARYOTES [J].

ELDER, JF ;

TURNER, BJ .

QUARTERLY REVIEW OF BIOLOGY, 1995, 70 (03) :297-320

[7]

Hall TA., 1999, NUCL ACIDS S SERIES, V41, P95, DOI DOI 10.14344/IOC.ML.11.1

[8] Clustal W and clustal X version 2.0 [J].

Larkin, M. A. ;

Blackshields, G. ;

Brown, N. P. ;

Chenna, R. ;

McGettigan, P. A. ;

McWilliam, H. ;

Valentin, F. ;

Wallace, I. M. ;

Wilm, A. ;

Lopez, R. ;

Thompson, J. D. ;

Gibson, T. J. ;

Higgins, D. G. .

BIOINFORMATICS, 2007, 23 (21) :2947-2948

[9] SCAR markers and multiplex PCR-based identification of isomorphic species in the Anopheles dirus complex in Southeast Asia [J].

Manguin, S ;

Kengne, P ;

Sonnier, L ;

Harbach, RE ;

Baimai, V ;

Trung, HD ;

Coosemans, M .

MEDICAL AND VETERINARY ENTOMOLOGY, 2002, 16 (01) :46-54

[10] The second internal transcribed spacer of nuclear ribosomal DNA as a tool for Latin American anopheline taxonomy - A critical review [J].

Marrelli, Mauro Toledo ;

Sallum, Maria Anice Mureb ;

Marinotti, Osvaldo .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2006, 101 (08) :817-832

← 1 2 3 →