Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry

被引：72

作者：

McGrath, J. S. ^{[1
]}

Honrado, C. ^{[2
,3
]}

Spencer, D. ^{[2
,3
]}

Horton, B. ^{[4
]}

Bridle, H. L. ^{[1
]}

Morgan, H. ^{[2
,3
]}

机构：

[1] Heriot Watt Univ, Sch Engn & Phys Sci, Inst Biol Chem Biophys & Bioengn, Edinburgh EH14 4AS, Midlothian, Scotland

[2] Univ Southampton, Fac Phys Sci & Engn, Southampton SO17 1BJ, Hants, England

[3] Univ Southampton, Inst Life Sci, Southampton SO17 1BJ, Hants, England

[4] Moredun Sci, Pentlands Sci Pk,Bush Loan, Penicuik EH26 0PZ, Midlothian, Scotland

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

英国工程与自然科学研究理事会;

关键词：

CRYPTOSPORIDIUM-PARVUM OOCYSTS; IDENTIFICATION; INFECTIVITY; SEPARATION; VIABILITY; DISEASE;

D O I：

10.1038/s41598-017-02715-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

At present, there are few technologies which enable the detection, identification and viability analysis of protozoan pathogens including Cryptosporidium and/or Giardia at the single (oo) cyst level. We report the use of Microfluidic Impedance Cytometry (MIC) to characterise the AC electrical (impedance) properties of single parasites and demonstrate rapid discrimination based on viability and species. Specifically, MIC was used to identify live and inactive C. parvum oocysts with over 90% certainty, whilst also detecting damaged and/or excysted oocysts. Furthermore, discrimination of Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia, with over 92% certainty was achieved. Enumeration and identification of (oo) cysts can be achieved in a few minutes, which offers a reduction in identification time and labour demands when compared to existing detection methods.

引用

页数：11

共 53 条

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