A novel C-type lectin with microbiostatic and immune regulatory functions from Litopenaeus vannamei

被引:35
作者
Luo, Mengting [1 ,2 ,3 ]
Yang, Linwei [1 ,2 ,3 ]
Wang, Zi-ang [1 ,2 ,3 ]
Zuo, Hongliang [1 ,2 ,3 ]
Weng, Shaoping [1 ,2 ,3 ]
He, Jianguo [1 ,2 ,3 ,4 ]
Xu, Xiaopeng [1 ,2 ,3 ,4 ]
机构
[1] Sun Yat Sen Univ, Sch Life Sci, MOE Key Lab Aquat Prod Safety, State Key Lab Biocorurol, Guangzhou, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Inst Aquat Econ Anim, Guangzhou, Guangdong, Peoples R China
[3] Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou, Guangdong, Peoples R China
[4] South China Sea Resource Exploitat & Protect Coll, Guangzhou, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
C-type lectin; Microbiostatic activity; Immune regulation; Litopenaeus vannamei; SHRIMP; RECOGNITION; IDENTIFICATION; PATHWAY; BINDING; DOMAIN; RECEPTORS; PROTEIN; GENE; PROPHENOLOXIDASE;
D O I
10.1016/j.fsi.2019.07.047
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
C-type lectins (CTLs) are a group of lectins with at least one carbohydrate recognition domain (CRD), the binding of which to carbohydrates requires the presence of calcium ions. CTLs generally function as pattern recognition receptors (PRRs), essentially participating in innate immunity. In the current study, a novel CTL termed LvCTL5 was identified from Pacific white shrimp Litopenaeus vannamei, which shared sequence identities with other crustacean CTLs. LvCTL5 was highly expressed in hepatopancreas and could be activated by infection with bacteria, virus and fungi. The recombinant LvCTL5 protein purified from E. coli showed microbiostatic and agglutination activities against bacteria and fungi in vitro. Silencing of LvCTL5 in vivo could significantly affect expression of a series of immune effector genes and down-regulate the phagocytic activity of hemocytes. Compared with controls, the LvCTL5-silenced shrimp were highly susceptible to Vibrio parahaemolyticus and white spot syndrome virus (WSSV) infections. These suggest that LvCTL5 has microbiostatic and immune regulatory activities and is implicated in antiviral and antibacterial responses.
引用
收藏
页码:361 / 368
页数:8
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