Effects of Nano-hydroxyapatite/Poly(DL-lactic-co-glycolic acid) Microsphere-Based Composite Scaffolds on Repair of Bone Defects: Evaluating the Role of Nano-hydroxyapatite Content

被引：15

作者：

He, Shu ^{[1
]}

Lin, Kai-Feng ^{[1
]}

Sun, Zhen ^{[1
]}

Song, Yue ^{[1
]}

Zhao, Yi-Nan ^{[1
]}

Wang, Zheng ^{[1
]}

Bi, Long ^{[1
]}

Liu, Jian ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Orthopaed, 127 West Changle Rd, Xian 710032, Shaanxi, Peoples R China

来源：

ARTIFICIAL ORGANS | 2016年 / 40卷 / 07期

基金：

中国国家自然科学基金;

关键词：

Nano-hydroxyapatite; Poly(DL-lactic-co-glycolic acid); Microsphere-based composite; Bone tissue engineering; Bone regeneration; MESENCHYMAL STEM-CELLS; IN-VITRO; DEGRADATION CHARACTERISTICS; TISSUE REGENERATION; PLGA; BIOCOMPATIBILITY; MARROW; DIFFERENTIATION; ADHESION; INSULIN;

D O I：

10.1111/aor.12741

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The aim of the current study was to prepare microsphere-based composite scaffolds made of nano-hydroxyapatite (nHA)/poly (DL-lactic-co-glycolic acid) (PLGA) at different ratios and evaluate the effects of nHA on the characteristics of scaffolds for tissue engineering application. First, microsphere-based composite scaffolds made of two ratios of nHA/PLGA (nHA/PLGA=20/80 and nHA/PLGA=50/50) were prepared. Then, the effects of nHA on the wettability, mechanical strength, and degradation of scaffolds were investigated. Second, the biocompatibility and osteoinductivity were evaluated and compared by co-culture of scaffolds with bone marrow stromal stem cells (BMSCs). The results showed that the adhesion, proliferation, and osteogenic differentiation of BMSCs with nHA/PLGA (50/50) were better than those with nHA/PLGA (20/80). Finally, we implanted the scaffolds into femur bone defects in a rabbit model, then the capacity of guiding bone regeneration as well as the in vivo degradation were observed by micro-CT and histological examinations. After 4 weeks' implantation, there was no significant difference on the repair of bone defects. However, after 8 and 12 weeks' implantation, the nHA/PLGA (20/80) exhibited better bone formation than nHA/PLGA (50/50). These results suggested that a proper concentration of nHA in the nHA/PLGA composite should be taken into account when the composite scaffolds were prepared, which plays an important role in the biocompatibility, degradation rate and osteoconductivity.

引用

页码：E128 / E135

页数：8

共 29 条

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