Binding characteristics of SLE anti-DNA autoantibodies to modified DNA analogues

被引：0

作者：

Arjumand, S ^{[1
]}

Moinuddin ^{[1
]}

Ali, A ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Fac Med, Dept Biochem, Aligarh, Uttar Pradesh, India

来源：

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1997年 / 43卷 / 03期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Native calf thymus DNA was modified with furocoumarins and reactive oxygen species (ROS). The modifications were probed by UV & Fluorescence spectroscopy, thermal denaturation and hydroxyapatite chromatography. In CD spectroscopy changes in the ellipticity of the DNA molecule were observed as a result of modification. The binding specificity of naturally occurring anti-DNA antibodies with modified DNA molecules was assessed by ELISA and quantitative precipitin titration. The affinity of anti-DNA antibodies for modified conformers was found to be quite high.

引用

页码：643 / 653

页数：11

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