Flow cytometry vs cytomorphology for the detection of hematologic malignancy in body cavity fluids

被引：19

作者：

Cesana, Clara ^{[1
]}

Klersy, Catherine ^{[2
]}

Scarpati, Barbara ^{[1
]}

Brando, Bruno ^{[3
]}

Volpato, Elisabetta ^{[1
]}

Bertani, Giambattista ^{[1
]}

Faleri, Maurizio ^{[4
]}

Nosari, Annamaria ^{[1
]}

Cantoni, Silvia ^{[1
]}

Ferri, Ursula ^{[1
]}

Scampini, Linda ^{[1
]}

Barba, Claudia ^{[1
]}

Lando, Giuliana ^{[1
]}

Morra, Enrica ^{[1
]}

Cairoli, Roberto ^{[1
]}

机构：

[1] Osped Niguarda Ca Granda Hosp, Div Hematol, Dept Transfus Med, I-20162 Milan, Italy

[2] Fdn IRCCS Policlin San Matteo, Serv Biometry & Stat, Pavia, Italy

[3] Legnano Gen Hosp, Hematol Lab, Ctr Blood, Legnano, Italy

[4] Osped Niguarda Ca Granda Hosp, Div Surg Pathol & Cytogenet, I-20162 Milan, Italy

来源：

LEUKEMIA RESEARCH | 2010年 / 34卷 / 08期

关键词：

Bronchoalveolar lavage fluid; Cytomorphology; Flow cytometry; Hematologic malignancy; Non-Hodgkin lymphoma; Serous effusion; OCCULT LEPTOMENINGEAL DISEASE; MINIMAL RESIDUAL DISEASE; BRONCHOALVEOLAR LAVAGE; LYMPHOCYTES; LYMPHOMAS; CELLS; EFFUSIONS; DIAGNOSIS;

D O I：

10.1016/j.leukres.2010.02.008

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Flow cytometry and cytomorphology results on 92 body cavity fluids [61 effusions and 31 bronchoalveolar lavage fluids (BALF)] from hematologic malignancy were compared with retrospective clinical outcome. We observed double true positive/negative results in 67 cases (73%), and double false negative results in 2 cases (2%). Immunophenotyping accounted for true positive/negative results in 22 out of 23 mismatched cases (25%), and retained significantly higher accuracy than that of cytomorphology especially in effusions and differentiated lymphoma. In BALF analysis, immunophenotyping and cytomorphology sensitivity was 75% and 0%, respectively. Flow cytometry retains the highest accuracy in detecting neoplastic cells in body cavity fluids. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1027 / 1034

页数：8

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