Development and validation of a UPLC-MS/MS method for the simultaneous determination of paritaprevir and ritonavir in rat liver

被引：12
作者：
Ocque, Andrew J. [1 ]
Hagler, Colleen E. [1 ]
Difrancesco, Robin [1 ]
Woolwine-Cunningham, Yvonne [2 ]
Bednasz, Cindy J. [1 ]
Morse, Gene D. [1 ]
Talal, Andrew H. [3 ,4 ]
机构：
[1] Univ Buffalo, New York State Ctr Excellence Bioinformat & Life, Sch Pharm & Pharmaceut Sci, Translat Pharmacol Res Core,Dept Pharm Practice, Buffalo, NY USA
[2] Univ Buffalo, Clin & Translat Res Ctr, Buffalo, NY USA
[3] Univ Buffalo, Jacobs Sch Med, Div Gastroenterol & Hepatol, Buffalo, NY USA
[4] Univ Buffalo, Jacobs Sch Med, Ctr Clin & Res Liver Dis, Buffalo, NY USA
来源：
BIOANALYSIS | 2016年 / 8卷 / 13期
关键词：
core needle biopsy; fine needle aspirate; liver; paritaprevir; ritonavir; UPLC-MS/MS; validation; PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; HIV PROTEASE INHIBITORS; FINE-NEEDLE ASPIRATION; HUMAN PLASMA; QUANTITATIVE-DETERMINATION; CORE BIOPSY; STABILITY; QUANTIFICATION; BIOANALYSIS;
D O I：
10.4155/bio-2016-0040
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Aim: Determination of paritaprevir and ritonavir in rat liver tissue samples. Results: We successfully validated a UPLC-MS/MS method to measure paritaprevir and ritonavir in rat liver using deuterated internal standards (d8-paritapervir and d6-ritonavir). The method is linear from 20 to 20,000 and 5 to 10,000 pg on column for paritaprevir and ritonavir, respectively, and is normalized per milligram tissue. Interday and intraday variability ranged from 0.591 to 5.33% and accuracy ranged from -6.68 to 10.1% for quality control samples. The method was then applied to the measurement of paritaprevir and ritonavir in rat liver tissue samples from a pilot study. Conclusion: The validated method is suitable for measurement of paritaprevir and ritonavir within rat liver tissue samples for PK studies.
引用
收藏
页码：1353 / 1363
页数：11
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