Chemical composition and evaluation of the antinociceptive, antioxidant and antimicrobial effects of essential oil from Hymenaea cangaceira (Pinto, Mansano & Azevedo) native to Brazil: A natural medicine

被引:38
作者
de Veras, Bruno Oliveira [1 ]
Melo de Oliveira, Maria Betania [2 ]
da Silva Oliveira, Fernanda Granja [3 ]
dos Santos, Yago Queiroz [4 ]
Saturnino de Oliveira, Joao Ricardhis [2 ]
de Menezes Lima, Vera Lucia [2 ]
Guedes da Silva Almeida, Jackson Roberto [3 ]
do Amaral Ferraz Navarro, Daniela Maria [5 ]
de Oliveira Farias de Aguiar, Julio Cesar Ribeiro [5 ]
Aguiar, Jaciana dos Santos [6 ]
Gorlach-Lira, Krystyna [7 ]
Dias de Assis, Caio Rodrigo [2 ]
da Silva, Marcia Vanusa [2 ]
de Souza Lopes, Ana Catarina [1 ]
机构
[1] Univ Fed Pernambuco, Lab Microbiol, Postgrad Trop Med, BR-50670420 Recife, PE, Brazil
[2] Univ Fed Pernambuco, Lab Nat Prod, Dept Biochem, BR-50670420 Recife, PE, Brazil
[3] Fed Univ Sao Francisco Valley, Lab Nat Prod, Postgrad Biotechnol, BR-56304917 Petrolina, PE, Brazil
[4] Univ Fed Rio Grande do Norte, Lab Prot & Bioact Peptides, Dept Biochem, BR-59078970 Natal, RN, Brazil
[5] Univ Fed Pernambuco, Ctr Exact & Nat Sci, Dept Chem, BR-50670420 Recife, PE, Brazil
[6] Univ Fed Pernambuco, Lab Nat Prod, Postgrad Biotechnol, BR-50670420 Recife, PE, Brazil
[7] Univ Fed Paraiba, Lab Biol Mol Microorganisms, Dept Mol & Cellular Biol, BR-58059900 Joao Pessoa, Paraiba, Brazil
关键词
Fabaceae; Essential oil; Antimicrobial activity; Antioxidant activity; Antinociceptive; ANTIINFLAMMATORY PROPERTIES; PHENOLIC-COMPOUNDS; ALPHA-HUMULENE; FORMALIN TEST; L; LEAVES; ANTIBACTERIAL; SESQUITERPENE; CONSTITUENTS; VALIDATION;
D O I
10.1016/j.jep.2019.112265
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Ethnopharmacological relevance: Hymenaea cangaceira Pinto, Mansano & Azevedo (Fabaceae) is a Brazilian medicinal plant widely known as "Jatoba". In folk medicine, it is used to treat infections, respiratory problems, rheumatism, antitumoral, inflammation and pain, however, no activity has been scientifically validated. Aim of the study: This study investigated chemical composition of essential oil from Hymenaea cangaceira (EOHc), antimicrobial, antinociceptive and antioxidant activities besides protection against DNA damage and hemolysis. Material and methods: The essential oil was obtained by hydrodistillation, and characterized by GC-MS and GC-FID. The evaluation of antimicrobial activity was performed by microdilution method. The evaluation of the antioxidant activity was performed using the radicals DPPH, ABTS, O-2(-) and OH-, and the protection of DNA damage using plasmid pBR322. Different experimental models were used to evaluate the antinociceptive effect (acetic acid and formalin), and evaluate the mechanisms of action involved with pharmacological antagonists (naloxone, atropine and gibenclamide) in mice. The essential oil was evaluated for hemolysis on human erythrocytes. Results: The extraction of EOHc showed a yield of 0.18% on a dry basis, presenting high content of hydrocarbon sesquiterpenes (79.04%), high antioxidant activity and protect DNA from damage, besides presenting antifungal and antibacterial activity against Gram-positive and Gram-negative bacteria in vitro. It was found that the essential oil had no acute toxicity in mice up to 5000 mg/kg oral administration (o.a.), in addition to no hemolysis on human erythrocytes. The reduction of antinociceptive activity was 75%, with the opioid system as the mechanism of action. Conclusion: Our results validate the main activities by the traditional use attributed to H. cangaceira for antimicrobial and analgesic activity. In addition, the oil has a potent antioxidant activity, protecting the body against oxidative stress damage, adding new value to an endemic species not known to the industry.
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页数:9
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