Stable Single-Walled Carbon Nanotube-Streptavidin Complex for Biorecognition

被引：26

作者：

Liu, Zunfeng ^{[1
]}

Galli, Federica ^{[2
]}

Janssen, Kjeld G. H. ^{[3
]}

Jiang, Linhua ^{[1
]}

van der Linden, Heiko J. ^{[3
]}

de Geus, Daniel C. ^{[1
]}

Voskamp, Patrick ^{[1
]}

Kuil, Maxim E. ^{[1
]}

Olsthoorn, Rene C. L. ^{[4
]}

Oosterkamp, Tjerk H. ^{[2
]}

Hankemeier, Thomas ^{[3
]}

Abrahams, Jan Pieter ^{[1
]}

机构：

[1] Leiden Univ, Gorlaeus Labs, Dept Biophys Struct Chem, Leiden Inst Chem, NL-2300 RA Leiden, Netherlands

[2] Leiden Univ, Leiden Inst Phys, NL-2333 CA Leiden, Netherlands

[3] Leiden Univ, Dept Analyt Biosci, NL-2300 RA Leiden, Netherlands

[4] Leiden Univ, Leiden Inst Chem, LIC Mol Genet, NL-2300 RA Leiden, Netherlands

来源：

JOURNAL OF PHYSICAL CHEMISTRY C | 2010年 / 114卷 / 10期

关键词：

CROSS-LINKED MACROMOLECULE; SIZE DETERMINATION; PROTEINS; IMMOBILIZATION; TRANSPORTERS; FUNCTIONALIZATION; DISPERSABILITY; SOLUBILIZATION; NANOPARTICLES; PURIFICATION;

D O I：

10.1021/jp911441d

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A novel method is described for preparing single walled carbon nanotube (SWNT)-streptavidin complexes via the biotin-streptavidin recognition. The complex shows stability in 18 days, strong biotin recognition capability, and excellent loading capacity (about I streptavidin tetramer per 20 nm of SWNT). capturing biotinylated DNA, fluorophores, and Au nanoparticles (NPs) on the SWNT-streptavidin complexes demonstrates their usefulness as a docking matrix, for instance for electron microscopy Studies, a technique requiring if virtually electron transparent Support.

引用

页码：4345 / 4352

页数：8

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