Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients

被引：14

作者：

Zida, Sylvie ^{[1
,2
,3
]}

Tuaillon, Edouard ^{[2
,3
]}

Barro, Makoura ^{[4
]}

Franchard, Arnaud Kwimatouo Lekpa ^{[1
]}

Kagone, Therese ^{[1
]}

Nacro, Boubacar ^{[4
]}

Ouedraogo, Abdoul Salam ^{[5
]}

Bollore, Karine ^{[2
,3
]}

Sanosyan, Armen ^{[2
,3
]}

Plantier, Jean-Christophe ^{[6
]}

Meda, Nicolas ^{[1
]}

Sangare, Lassana ^{[5
]}

Rouzioux, Christine ^{[7
]}

Rouet, Francois ^{[8
]}

Kania, Dramane ^{[1
]}

机构：

[1] Ctr MURAZ, Dept Sci Biomed, Lab Virol, Bobo Dioulasso, Burkina Faso

[2] Univ Montpellier, INSERM, U1058, F-34059 Montpellier, France

[3] CHRU Montpellier, Montpellier, France

[4] CHU Sanou Souro, Dept Pediat, Bobo Dioulasso, Burkina Faso

[5] CHU Yalgado Ouedraogo, Lab Bacteriol Virol, Ouagadougou, Burkina Faso

[6] CHRU Charles Nicole, Lab Virol, Rouen, France

[7] Univ Paris 05, Equipe Accueil 7327, Paris, France

[8] Inst Pasteur Cambodge, Unite VIH Hepatites, Phnom Penh, Cambodia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2016年 / 54卷 / 06期

关键词：

VIRAL LOAD; AFRICA; ASSAYS;

D O I：

10.1128/JCM.03372-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The impact of HIV-1 DNA coamplification during HIV-1 RNA quantification on dried blood spots (DBS) was explored. False-positive HIV RNA detection (22/62, 35%) was associated with high HIV-1 DNA levels. Specificity of HIV-1 RNA assays on DBS should be evaluated following manufacturer protocols on samples with HIV-1 DNA levels of >= 1,000 copies/10(6) peripheral blood mononuclear cells.

引用

页码：1641 / 1643

页数：3

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