Influence of agricultural practices and seasons on the abundance and community structure of culturable pseudomonads in soils under no-till management in Argentina

被引:15
作者
Cecilia Agaras, Betina [1 ]
Gabriel Wall, Luis [1 ]
Valverde, Claudio [1 ]
机构
[1] Univ Nacl Quilmes, Dept Ciencia & Tecnol, Lab Bioquim Microbiol & Interacc Biol Suelo, Buenos Aires, DF, Argentina
关键词
Pseudomonas; Soil microbiology; Rhizosphere; No-tillage; Culturable methods; Agricultural treatments; BACTERIAL COMMUNITY; 2,4-DIACETYLPHLOROGLUCINOL-PRODUCING PSEUDOMONAS; MICROBIAL-POPULATIONS; RHIZOSPHERE SOIL; PLANT; DIVERSITY; MAIZE; FLUORESCENS; ENUMERATION; SURVIVAL;
D O I
10.1007/s11104-014-2095-8
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Members of the genus Pseudomonas are common inhabitants of rhizospheres and soils, and it is known that soil types and crop species influence their population density and structure. 20 x 10(6) ha are cultivated under no-tillage in Argentina and there is a need to find new biologically-based soil quality indexes to distinguish between sustainable and non-sustainable agricultural practices. Pseudomonads abundance and community structure were analyzed in no-till soils with different agricultural practices, in productive fields along 400 km of Argentinean Pampas. We sampled soils and root systems from agricultural plots in which sustainable or non-sustainable agricultural practices have been applied. Samples were collected in summer and winter during 2010 and 2011. Culturable fluorescent and total pseudomonads were enumerated by plating on Gould's selective medium S1. Colonies from these plates served as DNA source to carry out PCR-RFLP community structure analysis of the pseudomonads-specific marker genes oprF and gacA. Abundance of total and fluorescent culturable pseudomonads in bulk soils was influenced by seasonal changes and agricultural practices. Rhizospheric counts from the same crop were affected by agricultural treatments. Also, crop species influenced pseudomonads density in the rhizosphere. Combined PCR-RFLP profile of both genes showed a seasonal grouping of samples. Sustainable soil management seems to promote pseudomonads development in soils, favoring root colonization of crops from those plots. Crop species influence total pseudomonads load of rhizospheres and its community structure. Total or relative pseudomonads load could function as soil quality indicator of good agricultural practices.
引用
收藏
页码:117 / 131
页数:15
相关论文
共 75 条
[1]  
AAPRESID, 2013, CERT AGR
[2]  
AAPRESID, 2013, MAN BUEN PRACT AGR I, P57
[3]   Microbial communities and enzyme activities in soils under alternative crop rotations compared to wheat-fallow for the Central Great Plains [J].
Acosta-Martinez, Veronica ;
Mikha, Maysoon M. ;
Vigil, Merle F. .
APPLIED SOIL ECOLOGY, 2007, 37 (1-2) :41-52
[4]   Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina [J].
Agaras, Betina ;
Wall, Luis G. ;
Valverde, Claudio .
APPLIED SOIL ECOLOGY, 2012, 61 :305-319
[5]  
[Anonymous], 1904, Arb. Der Dtsch. Landwirtsch. Ges, DOI DOI 10.12691/AEES-1-6-1
[6]   Soil quality indicators under continuous cropping systems in the Argentinean Pampas [J].
Aparicio, Virginia ;
Costa, Jose Luis .
SOIL & TILLAGE RESEARCH, 2007, 96 (1-2) :155-165
[7]   Enumeration of total bacteria and bacteria with genes for proteolytic activity in pure cultures and in environmental samples by quantitative PCR mediated amplification [J].
Bach, HJ ;
Tomanova, J ;
Schloter, M ;
Munch, JC .
JOURNAL OF MICROBIOLOGICAL METHODS, 2002, 49 (03) :235-245
[8]   The role of root exudates in rhizosphere interations with plants and other organisms [J].
Bais, Harsh P. ;
Weir, Tiffany L. ;
Perry, Laura G. ;
Gilroy, Simon ;
Vivanco, Jorge M. .
ANNUAL REVIEW OF PLANT BIOLOGY, 2006, 57 :233-266
[9]   Impact of plant species and site on rhizosphere-associated fungi antagonistic to Verticillium dahliae kleb [J].
Berg, G ;
Zachow, C ;
Lottmann, J ;
Götz, M ;
Costa, R ;
Smalla, K .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (08) :4203-4213
[10]   Plant species and soil type cooperatively shape the structure and function of microbial communities in the rhizosphere [J].
Berg, Gabriele ;
Smalla, Kornelia .
FEMS MICROBIOLOGY ECOLOGY, 2009, 68 (01) :1-13