Enhanced Wound Healing Activity of Undenatured Type I Collagen Isolated from Discarded Skin of Black Sea Gilthead Bream (Sparus aurata) Conditioned as 3D Porous Dressing

被引：10

作者：

Gaspar-Pintiliescu, Alexandra ^{[1
]}

Anton, Elena Daniela ^{[1
]}

Iosageanu, Andreea ^{[1
]}

Berger, Daniela ^{[2
]}

Matei, Cristian ^{[2
]}

Mitran, Raul-Augustin ^{[3
]}

Negreanu-Pirjol, Ticuta ^{[4
]}

Craciunescu, Oana ^{[1
]}

Moldovan, Lucia ^{[1
]}

机构：

[1] Natl Inst R&D Biol Sci, Dept Cellular & Mol Biol, 296 Bucuresti, Bucharest 060031, Romania

[2] Univ Politehn Bucuresti, Fac Appl Chem & Mat Sci, 1-7 Bucuresti,Polizu St, Bucharest 011061, Romania

[3] Romanian Acad, Ilie Murgulescu Inst Phys Chem, Dept Oxide Cpds & Mat Sci, 202 Bucuresti, Bucharest 060021, Romania

[4] Ovidius Univ Constanta, Fac Pharm, 1 Univ Alley, Constanta 900470, Romania

来源：

CHEMISTRY & BIODIVERSITY | 2021年 / 18卷 / 08期

关键词：

biomaterials; proteins; biological activity; marine collagen; wound healing; CARBON-DIOXIDE; EXTRACTION; BIOCOMPATIBILITY; ACID; PEPTIDES; PROTEINS;

D O I：

10.1002/cbdv.202100293

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acid-soluble, undenatured, type I collagen (BSC) isolated, for the first time, from gilthead bream skin and the novel fabricated 3D porous wound dressing were analyzed for physicochemical and biological properties, in order to offer a safe alternative to commercial bovine collagen (BC) products. SDS-polyacrylamide analysis confirmed the purity of BSC preparation. The hydroxyproline content and temperature of denaturation of BSC were lower than those of BC, in accordance with the structural data recorded by FT-IR spectroscopy. However, certain concentrations of BSC stimulated the cell metabolism of L929 fibroblasts in a higher proportion than BC. The 3D wound dressing presented high porosity and low surface hydrophobicity that could help cell attachment and growth. The rapid biodegradation of BSC wound dressing could explain the improved in vitro cell migration and wound closure rate. In conclusion, the skin of gilthead bream from the Black Sea coast represented a valuable source for the biomedical industry, providing biocompatible, biodegradable collagen and 3D porous wound dressing, as novel material with enhanced wound healing activity.

引用

页数：14

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