Proteomic and N-Terminomic TAILS Analyses of Human Alveolar Bone Proteins: Improved Protein Extraction Methodology and LysargiNase Digestion Strategies Increase Proteome Coverage and Missing Protein Identification

被引:17
作者
Bell, Peter A. [1 ,2 ]
Solis, Nestor [1 ,2 ]
Kizhakkedathu, Jayachandran N. [1 ,3 ]
Matthew, Ian [2 ]
Overall, Christopher M. [1 ,4 ]
机构
[1] Univ British Columbia, Life Sci Ctr, Ctr Blood Res, Vancouver, BC, Canada
[2] Univ British Columbia, Fac Dent, Dept Oral Biol & Med Sci, Vancouver, BC, Canada
[3] Univ British Columbia, Dept Pathol & Lab Med, Vancouver, BC, Canada
[4] Univ British Columbia, Fac Med, Dept Biochem & Mol Biol, Vancouver, BC, Canada
基金
加拿大创新基金会; 加拿大健康研究院;
关键词
bone proteomics; matrisome; HPP; missing protein; PE1; multienzyme; LysargiNase; mirror protease; pannexin-3; anoctamin-5; GLUTAMINYL CYCLASE; STATISTICAL-MODEL; DATABASE; ASSOCIATION; PEPTIDES; PROJECT; TANDEM; TISSUE; MS/MS;
D O I
10.1021/acs.jproteome.9b00445
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
With 2129 proteins still classified by the Human Proteome Organisation Human Proteome Project (HPP) as "missing" without compelling evidence of protein existence (PE) in humans, we hypothesized that in-depth proteomic characterization of tissues that are technically challenging to access and extract would yield evidence for tissue-specific missing proteins. Paradoxically, although the skeleton is the most massive tissue system in humans, as one of the poorest characterized by proteomics, bone falls under the HPP umbrella term as a "rare tissue". Therefore, we aimed to optimize mineralized tissue protein extraction methodology and workflows for proteomic and data analyses of small quantities of healthy young adult human alveolar bone. Osteoid was solubilized by GuHCl extraction, with hydroxyapatite-bound proteins then released by ethylenediaminetetraacetic acid demineralization. A subsequent GuHCl solubilization extraction was followed by solid-phase digestion of the remaining insoluble cross-linked protein using trypsin and then 6 M urea dissolution incorporating LysC digestion. Bone extracts were digested in parallel using trypsin, LysargiNase, AspN, or GluC prior to liquid chromatography-mass spectrometry analysis. Terminal Amine Isotopic Labeling of Substrates was used to purify semitryptic peptides, identifying natural and proteolytic-cleaved neo N-termini of bone proteins. Our strategy enabled complete solubilization of the organic bone matrix leading to extensive categorization of bone proteins in different bone matrix extracts, and hence matrix compartments, for the first time. Moreover, this led to the high confidence identification of pannexin-3, a "missing protein", found only in the insoluble collagenous matrix and revealed for the first time by trypsin solid-phase digestion. We also found a singleton proteotypic peptide of another missing protein, meiosis inhibitor protein 1. We also identified 17 proteins classified in neXtprot as PE1 based on evidence other than from MS, termed non-MS PE1 proteins, including >= 9-mer proteotypic peptides of four proteins.
引用
收藏
页码:4167 / 4179
页数:13
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