On-site environmental DNA detection of species using ultrarapid mobile PCR

被引:20
作者
Doi, Hideyuki [1 ]
Watanabe, Takeshi [2 ]
Nishizawa, Naofumi [3 ]
Saito, Tatsuya [4 ]
Nagata, Hisao [3 ]
Kameda, Yuichi [3 ]
Maki, Nobutaka [2 ]
Ikeda, Kousuke [2 ]
Fukuzawa, Takashi [3 ]
机构
[1] Univ Hyogo, Grad Sch Informat Sci, Kobe, Hyogo, Japan
[2] Pacific Consultants Co Ltd, Tokyo, Japan
[3] GO FOTON Inc, Tsukuba, Ibaraki, Japan
[4] Univ Hyogo, Grad Sch Simulat Studies, Kobe, Hyogo, Japan
关键词
conservation genetics; distribution; environmental DNA; fish;
D O I
10.1111/1755-0998.13448
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Molecular methods, including those for environmental DNA (eDNA), provide essential information for biological and conservation sciences. Molecular measurements are often performed in the laboratory, which limits their scope, especially for rapid on-site analysis. eDNA methods for species detection provide essential information for the management and conservation of species and communities in various environments. We have developed an innovative novel method for on-site eDNA measurements using an ultrarapid mobile PCR platform. We tested the ability of our method to detect the distribution of silver carp, Hypophthalmichthys molitrix, in rivers and lakes. Our method reduced the measurement time to 30 min and provided high detectability of aquatic organisms compared to national observation surveys that use multiple fishing nets and laboratory extraction/detection using a benchtop qPCR platform. Our on-site eDNA method can be immediately applied to various taxa and environments.
引用
收藏
页码:2364 / 2368
页数:5
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