Genistein inhibits chondrogenic differentiation and mineralization of ATDC5 cells

被引：4
|
作者：
Kitagawa, Aya [1 ]
Yamaguchi, Mana [1 ]
Fujiwara, Aki [1 ,3 ]
Shimizu, Muneshige [2 ]
Takahashi, Azusa [1 ,4 ]
Sone, Hideyuki [1 ]
Kamiyama, Shin [1 ]
机构：
[1] Univ Niigata Prefecture, Fac Human Life Studies, Dept Hlth & Nutr, Higashi Ku, 471 Ebigase, Niigata 9508680, Japan
[2] Tokai Univ, Fac Marine Sci & Technol, Dept Fisheries, 3-20-1 Orido, Shimizu, Shizuoka 4248610, Japan
[3] Osaka City Univ, Grad Sch Human Life Sci, Dept Mol & Biochem Nutr, Sumiyoshi Ku, 3-3-138 Sugimoto, Osaka 5588585, Japan
[4] Hokkaido Univ, Home Econ & Technol Educ, Kita Ku, Ainosato 5-3, Sapporo, Hokkaido 002E8502, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2021年 / 566卷
基金：
日本学术振兴会;
关键词：
Genistein; Daidzein; Phytoestrogen; Isoflavone; Chondrogenesis; ATDC5; BONE LOSS; IN-VITRO; PHYTOESTROGENS; CARTILAGE; ESTROGEN; SOY; PROLIFERATION; SOX9; METAANALYSIS; ISOFLAVONES;
D O I：
10.1016/j.bbrc.2021.05.101
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Isoflavones are phytoestrogens abundant in leguminous crops and are used to prevent a variety of hormonal disorders. In the present study, the effects of genistein and daidzein on the chondrogenic differentiation of ATDC5 cells were investigated. Genistein (10 mu M) treatment markedly reduced production of sulfated proteoglycans and collagen fibers in the ATDC5 cells. Genistein suppressed the expression of genes involved in chondrocyte differentiation such as Sox9, Col2a1, Col10a1, Acan, and Tgfb1. Additionally, genistein significantly decreased calcium deposition in ATDC5 cells during chondrogenic differentiation; however, it increased calcification under non-chondrogenic mineralizing conditions. Daidzein exhibited a similar effect of suppressing chondrogenesis in ATDC5 cells, although its efficacy was 10-times lower than that of genistein. These findings suggest that a high concentration of genistein inhibits chondrogenesis and chondrogenic mineralization, whereas it enhances non-chondrogenic mineralization. (C) 2021 Elsevier Inc. All rights reserved.
引用
收藏
页码：123 / 128
页数：6
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