Exogenous application of poly-γ-glutamic acid enhances stress defense in Brassica napus L. seedlings by inducing cross-talks between Ca2+, H2O2, brassinolide, and jasmonic acid in leaves

Poly-gamma-glutamic acid (gamma-PGA) is a microbe-secreted isopeptide shown to promote growth and enhance crop stress tolerance. However, its downstream signaling pathways are unknown. Here, we studied gamma-PGA-induced tolerance to salt and cold stresses. Pretreatment with gamma-PGA contributed to enhance stress tolerance of canola seedlings by promoting proline accumulation and total antioxidant capacity (T-AOC) improvement. Further, Ca2+, H2O2, brassinolide, and jasmonic acid were found to be involved in the gamma-PGA-induced process. First, using signal blockers, we concluded that gamma-PGA activated Ca2+ fluctuations in canola seedling leaves. Second, the activated Ca2+ further elicited H2O2 production by Ca2+-binding proteins CBL9, CPK4, and CPK5. Third, the H2O2 signal promoted brassinolide and jasmonic acid biosynthesis by upregulating key genes (DWF4 and LOX2, respectively) for synthesizing these compounds. Lastly, brassinolide and jasmonic acid increased H2O2 which promoted praline accumulation and T-AOC improvement and further enhanced Ca2+-binding proteins including CaM, CBL10, and CPK9. (C) 2017 Published by Elsevier Masson SAS.