A carnosine intervention study in overweight human volunteers: bioavailability and reactive carbonyl species sequestering effect

被引：61

作者：

Regazzoni, Luca ^{[1
]}

de Courten, Barbora ^{[2
,3
]}

Garzon, Davide ^{[1
]}

Altomare, Alessandra ^{[1
]}

Marinello, Cristina ^{[1
]}

Jakubova, Michaela ^{[4
]}

Vallova, Silvia ^{[4
]}

Krumpolec, Patrik ^{[5
]}

Carini, Marina ^{[1
]}

Ukropec, Jozef ^{[5
]}

Ukropcova, Barbara ^{[4
,5
]}

Aldini, Giancarlo ^{[1
]}

机构：

[1] Univ Milan, Dept Pharmaceut Sci, Milan, Italy

[2] Sch Publ Hlth & Prevent Med, Monash Ctr Hlth Res & Implementat, Melbourne, Vic, Australia

[3] Monash Hlth, Diabet & Vasc Med Unit, Locked Bag 29, Clayton, Vic 3168, Australia

[4] Comenius Univ, Fac Med, Inst Pathol Physiol, Bratislava, Slovakia

[5] Slovak Acad Sci, Inst Expt Endocrinol, Biomed Res Ctr, Bratislava, Slovakia

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

PROTEIN CARBONYLATION; RENAL-DISEASE; ADDUCTS; ALBUMIN; DETOXIFICATION; METABOLISM; STRESS; CYSTEINYLATION; HISTIDINE; PRODUCTS;

D O I：

10.1038/srep27224

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Carnosine is a natural dipeptide able to react with reactive carbonyl species, which have been recently associated with the onset and progression of several human diseases. Herein, we report an intervention study in overweight individuals. Carnosine (2 g/day) was orally administered for twelve weeks in order to evaluate its bioavailability and metabolic fate. Two carnosine adducts were detected in the urine samples of all subjects. Such adducts are generated from a reaction with acrolein, which is one of the most toxic and reactive compounds among reactive carbonyl species. However, neither carnosine nor adducts have been detected in plasma. Urinary excretion of adducts and carnosine showed a positive correlation although a high variability of individual response to carnosine supplementation was observed. Interestingly, treated subjects showed a significant decrease in the percentage of excreted adducts in reduced form, accompanied by a significant increase of the urinary excretion of both carnosine and carnosine-acrolein adducts. Altogether, data suggest that acrolein is entrapped in vivo by carnosine although the response to its supplementation is possibly influenced by individual diversities in terms of carnosine dietary intake, metabolism and basal production of reactive carbonyl species.

引用

页数：13

共 41 条

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