Interleukin-4 Contributes to Degeneration of Dopamine Neurons in the Lipopolysaccharide-treated Substantia Nigra in vivo

被引：27

作者：

Bok, Eugene ^{[1
]}

Cho, Eun Ju ^{[2
]}

Chung, Eun Sook ^{[2
]}

Shin, Won-Ho ^{[1
]}

Jin, Byung Kwan ^{[2
]}

机构：

[1] Korea Inst Toxicol, Dept Predict Toxicol, Daejeon 34114, South Korea

[2] Kyung Hee Univ, Dept Biochem & Mol Biol, Sch Med, Seoul 02447, South Korea

来源：

EXPERIMENTAL NEUROBIOLOGY | 2018年 / 27卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

Interleukin-4; Parkinson disease; Substantia nigra; Dopaminergic neurons; Lipopolysaccharides; BLOOD-BRAIN-BARRIER; OXIDATIVE STRESS CONTRIBUTES; PARKINSONS-DISEASE; INDUCED NEURODEGENERATION; MICROGLIAL ACTIVATION; HIPPOCAMPAL-NEURONS; NADPH OXIDASE; MODEL; EXPRESSION; RECEPTOR;

D O I：

10.5607/en.2018.27.4.309

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The present study investigated the effects of interleukin (IL)-4 on dopamine (DA) neurons in the substantia nigra (SN) in vivo of lipopolysaccharide (LPS)-treated rat. Tyrosine hydroxylase immunohistochemistry showed a significant loss of nigral DA neurons at 3 and 7 day post-LPS. In parallel, IL-4 immunoreactivity was upregulated as early as 1 day, reached a peak at 3 day and remained elevated at 7 day post-LPS. IL-4 immunoreactivity was detected exclusively in microglia. IL-4 neutralizing antibody (NA) significantly increased survival of DA neurons in LPS-treated SN in vivo by inhibiting microglial activation and production of proinflammatory mediator such as IL-1 beta as assessed by immunihistochemical, RT-PCR and ELISA analysis, respectively. Accompanying neuroprotection are IL-4NA effects on decreased disruption of blood-brain barrier and astrocytes. The present data suggest that endogenously expressed IL-4 from reactive microglia may be involved in the neuropathological processes of degeneration of DA neurons occurring in Parkinson's disease.

引用

页码：309 / 319

页数：11

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