Factors affecting secondary somatic embryogenesis and embryo maturation in Morus alba L.

被引：34
|
作者：
Agarwal, S [1 ]
Kanwar, K [1 ]
Sharma, DR [1 ]
机构：
[1] Dr YS Parmar Univ Hort & Forestry, Dept Biotechnol, Solan 173230, HP, India
来源：
SCIENTIA HORTICULTURAE | 2004年 / 102卷 / 03期
关键词：
Morus alba; secondary somatic embryogenesis; embryo maturation;
D O I：
10.1016/j.scienta.2004.04.002
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
A procedure for optimum secondary embryogenesis and maturation of Morus alba somatic embryos is described. The primary somatic embryos were inoculated on MS medium with different plant growth regulators and complex additives in various combinations and concentrations. Best results were obtained on the medium supplemented with 0.05 mg 2,4-D + 0. 1 mg l(-1) BAP and 6% sucrose, where percentage of secondary embryogenesis was 98.93 with 17% well-matured cotyledonary embryos. On this medium, the embryogenic competence was maintained for more than a year by repeated subculture. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：359 / 368
页数：10
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