Chitinase-catalyzed synthesis of an alternatingly N-sulfonated chitin derivative

被引：17

作者：

Makino, Akira ^{[1
]}

Nagashima, Hideaki ^{[1
]}

Ohmae, Masashi ^{[1
]}

Kobayashi, Shiro ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Engn, Dept Chem Mat, Kyoto 6158510, Japan

来源：

BIOMACROMOLECULES | 2007年 / 8卷 / 01期

关键词：

D O I：

10.1021/bm0609240

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An alternatingly N-sulfonated chitin derivative (2) was synthesized via ring-opening polyaddition of an N-sulfonated chitobiose oxazoline derivative (1) catalyzed by chitinases from Bacillus sp. and Serratia marcescens. The polymerization proceeded homogeneously, providing 2 as a water-soluble polysaccharide in good yields with total control of regioselectivity and stereochemistry. M-n of 2 reached 1900 and 4180 by use of chitinases from Bacillus sp. and Serratia marcescens, which correspond to 8-10 (n = 4-5) and 18-20 (n = 9-10) saccharide units, respectively. These results indicate that M-n of 2 is controllable by selecting chitinases from different origins. It is considered that the C-2 position of the nonreducing unit in the oxazoline-type monomer is not deeply involved in the catalysis of chitinase.

引用

页码：188 / 195

页数：8

共 34 条

[1]

[Anonymous], 2001, STARCH-STARKE

[2] Family 18 chitinase-oligosaccharide substrate interaction:: subsite preference and anomer selectivity of Serratia marcescens chitinase A [J].