Paclitaxel induces apoptosisins human gastric carcinoma cells

被引：28

作者：

Zhou, HB ^{[1
]}

Zhu, JR ^{[1
]}

机构：

[1] Shandong Provincial Hosp, Dept Gastroenterol, Jinan 250052, Shandong, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2003年 / 9卷 / 03期

关键词：

D O I：

10.3748/wjg.v9.i3.442

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To investigate the apoptosis in gastric cancer cells induced by paclitaxel and the relation between this apoptosis and expression of Bcl 2 and Bax. METHODS: In in vitro experiments, MTT assay was used to determine the cell growth inhibitory rate. Transmission electron microscope and TUNEL staining method were used to quantitatively and qualitively detect the apoptosis status of gastric cancer cell line SGC 7901 before and after the paclitaxel treatment. Immunohistochemical staining was used to detect the expression of apoptosis-regulated gene Bcl-2 and Bax. RESULTS: Paclitaxel inhibited the growth of gastric cancer cell line SGC 7901 cells to undergo apoptosis with typically apoptotic characteristics, including morphological changes of chromatin condensation, chromatin cresent formation, nucleus fragmentation and apoptotic body formation. Paclitaxel could reduce the expression of apoptosis-regulated gene Bcl-2, and improve the expression of apoptosis regulated gene Bax. CONCLUSION: Paclitaxel able to induce the apoptosis in gastric cancer. This apoptosis-regulated Bcl-2 and up expression of apoptosis-regulated gene Bax.

引用

页码：442 / 445

页数：4

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