In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates

被引:32
作者
Turin-Moleavin, Ioana-Andreea [1 ]
Fifere, Adrian [1 ]
Lungoci, Ana-Lacramioara [1 ]
Rosca, Irina [1 ]
Coroaba, Adina [1 ]
Peptanariu, Dragos [1 ]
Nastasa, Valentin [2 ]
Pasca, Sorin-Aurelian [2 ]
Bostanaru, Andra-Cristina [2 ]
Mares, Mihai [2 ]
Pinteala, Mariana [1 ]
机构
[1] Petru Poni Inst Macromol Chem, Ctr Adv Res, Bionanoconjugates & Biopolymers Dept, 41A Grigore Ghica Voda Alley, Iasi 700487, Romania
[2] Ion Ionescu de la Brad Univ Agr Sci & Vet Med, Lab Antimicrobial Chemotherapy, 8 Sadoveanu Alley, Iasi 700489, Romania
关键词
core-shell magnetic nanoparticles; antioxidant activity; free radical scavengers; cerium oxide nanoparticles; CARGO-COMPLEXES; NANOPARTICLES; BIOCOMPATIBILITY; ANTIBACTERIAL; NANOMATERIALS; SPECTROSCOPY;
D O I
10.3390/nano9111565
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. This study presents the synthesis and biological characterisation of nanoconjugates based on nanoparticles of iron oxide interconnected with cerium oxide conjugates. Methods. The synthesis of magnetite-nanoceria nanoconjugates has been done in several stages, where the key to the process is the coating of nanoparticles with polyethyleneimine and its chemical activation-reticulation with glutaraldehyde. The nanoconjugates are characterised by several techniques, and the antioxidant activity was evaluated in vitro and in vivo. Results. Iron oxide nanoparticles interconnected with cerium oxide nanoparticles were obtained, having an average diameter of 8 nm. Nanoconjugates prove to possess superparamagnetic properties and the saturation magnetisation varies with the addition of diamagnetic components in the system, remaining within the limits of biomedical applications. In vitro free-radical scavenging properties of nanoceria are improved after the coating of nanoparticles with polyethylenimine and conjugation with magnetite nanoparticles. In vivo studies reveal increased antioxidant activity in all organs and fluids collected from mice, which demonstrates the ability of the nanoconjugates to reduce oxidative stress. Conclusion. Nanoconjugates possess magnetic properties, being able to scavenge free radicals, reducing the oxidative stress. The combination of the two properties mentioned above makes them excellent candidates for theranostic applications.
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页数:21
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