Method Development for Electrotransformation of Acidithiobacillus caldus

被引：15

作者：

Chen Linxu ^{[1
]}

Lin, Jianqun ^{[1
]}

Li, Bing ^{[1
]}

Lin, Jianqiang ^{[1
]}

Liu, Xiangmei ^{[1
]}

机构：

[1] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2010年 / 20卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Acidithiobacillus caldus; chemolithotrophic; electrotransformation; optimization; HIGH-EFFICIENCY TRANSFORMATION; THIOBACILLUS-CALDUS; ESCHERICHIA-COLI; ELECTROPORATION; FERROOXIDANS; THIOOXIDANS; CELLS; ACIDOPHILE; ATTACHMENT; PLASMIDS;

D O I：

10.4014/jmb.0905.05023

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Acidithiobacillus caldus is an acidophilic, chemolithotrophic bacterium that plays an important role in bioleaching. Gene transformation into A. caldus is difficult, and only the conjugation method was reported successful, which was a relatively sophisticated method. In this research, electrotransformation of A. caldus species was achieved for the first time using A. caldus Y-3 and plasmid pJRD215. Transformants were confirmed by colony PCR specific to the str gene on pJRD215, and the recovery of the plasmid from the presumptive transformants. Optimizations were made and the transformation efficiency was increased from 0.8 to 3.6 x 10(4) transformants/mu g plasmid DNA. The developed electrotransformation method was convenient in introducing foreign genes into A. caldus.

引用

页码：39 / 44

页数：6

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