A Carborane-Containing Fluorophore as a Stain of Cellular Lipid Droplets

被引：30

作者：

Wu, Andrew ^{[1
]}

Kolanowski, Jacek L. ^{[1
]}

Boumelhem, Badwi B. ^{[2
]}

Yang, Kylie ^{[1
]}

Lee, Rebecca ^{[1
]}

Kaur, Amandeep ^{[1
]}

Fraser, Stuart T. ^{[2
]}

New, Elizabeth J. ^{[1
]}

Rendina, Louis M. ^{[1
]}

机构：

[1] Univ Sydney, Sch Chem, Sydney, NSW 2006, Australia

[2] Univ Sydney, Sch Med Sci, Discipline Physiol, Sydney, NSW 2006, Australia

来源：

CHEMISTRY-AN ASIAN JOURNAL | 2017年 / 12卷 / 14期

关键词：

carboranes; cellular markers; coumarins; fluorophores; lipid droplets; CONFOCAL MICROSCOPY; FLUORESCENT-PROBE; COMPLEXES; COUMARIN; DYNAMICS; BIOLOGY; BORON;

D O I：

10.1002/asia.201700423

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The use of fluorescent markers and probes greatly enhances biological investigations but relies on the provision of an array of fluorophores with diverse properties. Herein we report a novel carborane-containing coumarin, 5, which is sufficiently lipophilic to localise in cellular lipid droplets. In non-polar solvents which show comparable polarities to those of a lipid environment, compound5 exhibits a fluorescence quantum yield two orders of magnitude greater than found in aqueous solvents, adding a further degree of selectivity to lipid droplet imaging. Compound5 can stain lipid droplets in ex vivo adipocytes as well as in cultured cells, and can be utilised in flow cytometry as well as confocal microscopy.

引用

页码：1704 / 1708

页数：5

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