A novel near-infrared fluorescent probe with a large stokes shift for the detection and imaging of biothiols

被引：78

作者：

Wang, Kai ^{[1
,2
]}

Leng, Taohua ^{[3
]}

Liu, Yajing ^{[4
]}

Wang, Chengyun ^{[1
,2
]}

Shi, Ping ^{[4
]}

Shen, Yongjia ^{[1
,2
]}

Zhu, Wei-Hong ^{[1
,2
]}

机构：

[1] East China Univ & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Key Lab Adv Mat, Shanghai 200237, Peoples R China

[2] East China Univ & Technol, Sch Chem & Mol Engn, Inst Fine Chem, Shanghai 200237, Peoples R China

[3] Shanghai Inst Qual Inspect & Tech Res, Natl Food Qual Supervis & Inspect Ctr Shanghai, Shanghai 200233, Peoples R China

[4] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 248卷

基金：

上海市自然科学基金; 中国国家自然科学基金;

关键词：

Isophorone-based dye; Near-infrared fluorescence; Large stokes shift; Biothiols; Cell imaging; HIGHLY SELECTIVE DETECTION; DIFFERENT EMISSION CHANNELS; TURN-ON FLUORESCENT; IN-VIVO; LIVING CELLS; THERANOSTIC PRODRUG; RECENT PROGRESS; GLUTATHIONE; CYSTEINE; HOMOCYSTEINE;

D O I：

10.1016/j.snb.2017.03.127

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, a dicyanoisophorone-based fluorophore, which showed an attractive near-infrared fluorescence as well as a favorable large stokes shift, was used to develop a novel probe (NIR-Probe 1) for effective detection of biothiols. It displayed distinct color changes and a turn-on NIR fluorescence response with a high off/on ratio toward biothiols. We demonstrated that NIR-Probe 1 exhibited fast sensing speed, high selectivity for biothiols and possessed the function of detecting low concentration of biothiols with impressive limit of detection: 12.1 nM for Cys, 14.5 nM for Hcy and 11.9 nM for GSH. Moreover, the utility of NIR-Probe 1 as a bioanalytical molecular tool was demonstrated by fluorescence imaging of biothiols in living cells.(C) 2017 Elsevier B.V. All rights reserved.

引用

页码：338 / 345

页数：8

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