Isolation and optimization of extracellular PHB depolymerase producer Aeromonas caviae Kuk1-(34) for sustainable solid waste management of biodegradable polymers

被引:17
作者
Amir, Mohammad [1 ]
Bano, Naushin [1 ]
Baker, Abu [1 ]
Zia, Qamar [2 ,3 ]
Banawas, Saeed [2 ,3 ,4 ]
Zaheer, Mohd Rehan [5 ]
Shariq, Mohammad [6 ]
Nawaz, Md Sarfaraz [7 ]
Khan, Mohd Farhan [5 ,8 ]
Azad, Z. R. Azaz Ahmad [9 ]
Gupta, Anamika [10 ]
Iqbal, Danish [3 ]
Roohi [1 ]
机构
[1] Integral Univ, Dept Bioengn, Prot Res Lab, Lucknow, Uttar Pradesh, India
[2] Majmaah Univ, Hlth & Basic Sci Res Ctr, Majmaah, Saudi Arabia
[3] Majmaah Univ, Coll Appl Med Sci, Dept Med Lab Sci, Majmaah, Saudi Arabia
[4] Oregon State Univ, Dept Biomed Sci, Corvallis, OR USA
[5] Gagan Coll Management & Technol, Dept Sci, Aligarh, Uttar Pradesh, India
[6] Jazan Univ, Fac Sci, Dept Phys, Jazan, Saudi Arabia
[7] Jazan Univ, Fac Sci, Dept Chem, Jazan, Saudi Arabia
[8] Aligarh Muslim Univ, ZH Coll Engn & Technol, Dept Mech Engn, Nano Solver Lab, Aligarh, Uttar Pradesh, India
[9] Aligarh Muslim Univ, Dept Postharvest Engn & Technol, Aligarh, Uttar Pradesh, India
[10] Aligarh Muslim Univ, Dept Chem, Aligarh, Uttar Pradesh, India
来源
PLOS ONE | 2022年 / 17卷 / 04期
关键词
POLY(3-HYDROXYBUTYRATE) DEPOLYMERASE; POLYHYDROXYBUTYRATE DEPOLYMERASE; MICROBIAL-DEGRADATION; PURIFICATION; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE); BACTERIA;
D O I
10.1371/journal.pone.0264207
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Bioplastics, synthesized by several microbes, accumulates inside cells under stress conditions as a storage material. Several microbial enzymes play a crucial role in their degradation. This research was carried to test the biodegradability of poly-beta-hydroxybutyrate (PHB) utilizing PHB depolymerase, produced by bacteria isolated from sewage waste soil samples. Potent PHB degrader was screened based on the highest zone of hydrolysis followed by PHB depolymerase activity. Soil burial method was employed to check their degradation ability at different incubation periods of 15, 30, and 45 days at 37 +/- 2 degrees C, pH 7.0 at 60% moisture with 1% microbial inoculum of Aeromonas caviae Kuk1-(34) (MN414252). Without optimized conditions, 85.76% of the total weight of the PHB film was degraded after 45 days. This degradation was confirmed with Fourier-transform infrared spectroscopy (FTIR) and Scanning electron microscope (SEM) analysis. The presence of bacterial colonies on the surface of the degraded film, along with crest, holes, surface erosion, and roughness, were visible. Media optimization was carried out in statistical mode using Plackett Burman (PB) and Central Composite Design (CCD) of Response Surface Methodology (RSM) by considering ten different factors. Analysis of Variance (ANOVA), Pareto chart, response surface plots, and F-value of 3.82 implies that the above statistical model was significant. The best production of PHB depolymerase enzyme (14.98 U/mL) was observed when strain Kuk1-(34) was grown in a media containing 0.1% PHB, K2HPO4 (1.6 gm/L) at 27 degrees C for seven days. Exploiting these statistically optimized conditions, the culture was found to be a suitable candidate for the management of solid waste, where 94.4% of the total weight of the PHB film was degraded after 45 days of incubation.
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页数:20
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