Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1-4 infections in wild rats from Lithuania

被引：17

作者：

Simanavicius, Martynas ^{[1
]}

Juskaite, Karolina ^{[1
,5
]}

Verbickaite, Arune ^{[1
,5
]}

Jasiulionis, Marius ^{[2
,6
]}

Tamosiunas, Paulius Lukas ^{[1
,5
]}

Petraityte-Burneikiene, Rasa ^{[1
,5
]}

Zvirbliene, Aurelija ^{[1
,5
]}

Ulrich, Rainer G. ^{[3
,4
,7
]}

Kucinskaite-Kodze, Indre ^{[1
,5
]}

机构：

[1] Vilnius Univ, Life Sci Ctr, Inst Biotechnol, Vilnius, Lithuania

[2] Nat Res Ctr, Lab Mammalian Ecol, Vilnius, Lithuania

[3] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Novel & Emerging Infect Dis, Greifswald, Germany

[4] German Ctr Infect Res DZIF, Partner Site Hamburg Luebeck Borstel Insel Riems, Braunschweig, Germany

[5] Sauletekio Al 7, LT-10257 Vilnius, Lithuania

[6] Akad 2, LT-08412 Vilnius, Lithuania

[7] Sudufer 10, D-17493 Greifswald, Germany

来源：

VETERINARY MICROBIOLOGY | 2018年 / 221卷

关键词：

Hepatitis E virus (HEV); Rat HEV; Genotype C1; SEROLOGICAL EVIDENCE; RT-PCR; NORVEGICUS; PARTICLES;

D O I：

10.1016/j.vetmic.2018.06.014

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rat hepatitis E virus (HEV) is an orthohepevirus which is related to other HEV found in humans and other mammals. It was first identified in Norway rats (Rattus norvegicus) from Germany in 2010, and later it has been detected in Black rats (Rattus rattus) and Norway rats from USA, China, Indonesia, Vietnam and many European countries. In this study, we describe molecular and serological investigations of Black and Norway rats trapped in Lithuania, Eastern Europe, for infections with rat HEV and human HEV genotypes 1-4. Rat HEV-specific real-time reverse transcription-PCR (RT-qPCR) analysis of rat liver samples revealed the presence of rat HEV in 9 of 109 (8.3%) samples. In contrast, a RT-qPCR specific for HEV genotypes 1-4 did not reveal any positive samples. A nested broad spectrum RT-PCR was used for a confirmation of rat HEV infection with a subsequent sequencing of the amplified rat HEV genome fragment. Phylogenetic analysis revealed a clustering of all newly identified rat HEV sequences with Norway rat-derived rat HEV sequences from Germany within the species Orthohepevirus C. An indirect ELISA using a yeast-expressed truncated rat HEV capsid protein variant revealed 31.2% seropositive samples indicating a high rate of rat HEV circulation in the rat population examined. In conclusion, the current investigation confirms rat HEV infections in Norway and Black rats in Lithuania, Eastern Europe, and the non persistent nature of HEV infection.

引用

页码：129 / 133

页数：5

共 24 条

[1] Seroprevalence study in forestry workers from eastern Germany using novel genotype 3-and rat hepatitis E virus-specific immunoglobulin G ELISAs [J].

Dremsek, Paul ;

Wenzel, Juergen J. ;

Johne, Reimar ;

Ziller, Mario ;

Hofmann, Joerg ;

Groschup, Martin H. ;

Werdermann, Sandra ;

Mohn, Ulrich ;

Dorn, Silvia ;

Motz, Manfred ;

Mertens, Marc ;

Jilg, Wolfgang ;

Ulrich, Rainer G. .

MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 2012, 201 (02) :189-200

[2] Rat hepatitis E virus: Geographical clustering within Germany and serological detection in wild Norway rats (Rattus norvegicus) [J].

Johne, Reimar ;

Dremsek, Paul ;

Kindler, Eveline ;

Schielke, Anika ;

Plenge-Boenig, Anita ;

Gregersen, Henrike ;

Wessels, Ute ;

Schmidt, Katja ;

Rietschel, Wolfram ;

Groschup, Martin H. ;

Guenther, Sebastian ;

Heckel, Gerald ;

Ulrich, Rainer G. .

INFECTION GENETICS AND EVOLUTION, 2012, 12 (05) :947-956

[3] Novel Hepatitis E Virus Genotype in Norway Rats, Germany [J].

Johne, Reimar ;

Heckel, Gerald ;

Plenge-Boenig, Anita ;

Kindler, Eveline ;

Maresch, Christina ;

Reetz, Jochen ;

Schielke, Anika ;

Ulrich, Rainer G. .

EMERGING INFECTIOUS DISEASES, 2010, 16 (09) :1452-1455

[4] Detection of a novel hepatitis E-like virus in faeces of wild rats using a nested broad-spectrum RT-PCR [J].

Johne, Reimar ;

Plenge-Boenig, Anita ;

Hess, Michael ;

Ulrich, Rainer G. ;

Reetz, Jochen ;

Schielke, Anika .

JOURNAL OF GENERAL VIROLOGY, 2010, 91 :750-758

[5] A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus [J].

Jothikumar, N ;

Cromeans, TL ;

Robertson, BH ;

Meng, XJ ;

Hill, VR .

JOURNAL OF VIROLOGICAL METHODS, 2006, 131 (01) :65-71

[6] Hepatitis E virus infection [J].

Kamar, Nassim ;

Izopet, Jacques ;

Pavio, Nicole ;

Aggarwal, Rakesh ;

Labrique, Alain ;

Wedemeyer, Heiner ;

Dalton, Harry R. .

NATURE REVIEWS DISEASE PRIMERS, 2017, 3

[7] Hepatitis e virus in Norway rats (Rattus norvegicus) captured around a pig farm [J].

Yuta Kanai ;

Satoshi Miyasaka ;

Sachiko Uyama ;

Sachiyo Kawami ;

Yuko Kato-Mori ;

Muneo Tsujikawa ;

Mikihiro Yunoki ;

Shoko Nishiyama ;

Kazuyoshi Ikuta ;

Katsuro Hagiwara .

BMC Research Notes, 5 (1)

[8]

Kumar S, 2016, MOL BIOL EVOL, V33, P1870, DOI [10.1093/molbev/msw054, 10.1093/molbev/msv279]

[9] Hepatitis E Virus Genotype 3 in Wild Rats, United States [J].

Lack, Justin B. ;

Volk, Kylie ;

Van den Bussche, Ronald A. .

EMERGING INFECTIOUS DISEASES, 2012, 18 (08) :1268-1273

[10] Susceptibility of laboratory rats against genotypes 1, 3, 4, and rat hepatitis E viruses [J].

Li, Tian-Cheng ;

Yoshizaki, Sayaka ;

Ami, Yasushi ;

Suzaki, Yuriko ;

Yasuda, Shumpei P. ;

Yoshimatsu, Kumiko ;

Arikawa, Jiro ;

Takeda, Naokazu ;

Wakita, Takaji .

VETERINARY MICROBIOLOGY, 2013, 163 (1-2) :54-61

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