Testicular cell adhesion molecule 1 (TCAM1) is not essential for fertility

被引:8
作者
Nalam, Roopa L. [1 ,2 ]
Lin, Yi-Nan [2 ,4 ]
Matzuk, Martin M. [1 ,2 ,3 ]
机构
[1] Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA
[2] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA
[3] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA
[4] Acad Sinica, Inst Biomed Sci, Taipei, Taiwan
关键词
Spermatocyte; Male fertility; Integral membrane protein; Transgenic mice; MALE-MICE; GENE; PROTEINS; SPERMATOGENESIS; IDENTIFICATION; ALIGNMENTS; EXPRESSION;
D O I
10.1016/j.mce.2009.09.010
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Testicular cell adhesion molecule 1 (Tcam1) is a testis-expressed gene that is evolutionarily conserved in most mammalian species. The putative location of TCAM1 on the cell surface makes it an attractive contraceptive target to study. We found that Tcam1 transcription is enriched in the adult testis, and in situ hybridization revealed that Tcam1 is expressed in pachytene to secondary spermatocytes. Immunofluorescence for TCAM1 protein showed strong expression along cell membranes of spermatocytes and weak localization to round spermatids. In light of this evidence, we hypothesized that TCAM1 interacts with an unknown receptor on the surface of Sertoli cells and that this interaction is important for germ cell-Sertoli cell interactions. However, Tcam1 knockout mice that we generated are fertile, and testis weights and sperm counts were not significantly altered. Therefore, we conclude that TCAM1 is not essential for male fertility or germ cell function in Mus musculus. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:246 / 253
页数:8
相关论文
共 29 条
  • [1] A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction
    Adams, DJ
    Quail, MA
    Cox, T
    van der Weyden, L
    Gorick, BD
    Su, Q
    Chan, WI
    Davies, R
    Bonfield, JK
    Law, F
    Humphray, S
    Plumb, B
    Liu, PT
    Rogers, J
    Bradley, A
    [J]. GENOMICS, 2005, 86 (06) : 753 - 758
  • [2] Albrecht U., 1997, MOL CELLULAR METHODS, P23
  • [3] [Anonymous], 2006, Molecular cell biology
  • [4] MAMMALIAN REPRODUCTION - AN ECOLOGICAL PERSPECTIVE
    BRONSON, FH
    [J]. BIOLOGY OF REPRODUCTION, 1985, 32 (01) : 1 - 26
  • [5] FRIEDRICH G, 1993, METHOD ENZYMOL, V225, P681
  • [6] The role of NK cell recognition of nectin and nectin-like proteins in tumor immunosurveillance
    Fuchs, Anja
    Colonna, Marco
    [J]. SEMINARS IN CANCER BIOLOGY, 2006, 16 (05) : 359 - 366
  • [7] TEX14 is essential for intercellular bridges and fertility in male mice
    Greenbaum, MP
    Yan, W
    Wu, MH
    Lin, YN
    Agno, JE
    Sharma, M
    Braun, RE
    Rajkovic, A
    Matzuk, MM
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (13) : 4982 - 4987
  • [8] Using multiple alignments to improve gene prediction
    Gross, SS
    Brent, MR
    [J]. JOURNAL OF COMPUTATIONAL BIOLOGY, 2006, 13 (02) : 379 - 393
  • [9] HIGGINS DG, 1989, COMPUT APPL BIOSCI, V5, P151
  • [10] PhyloPat: phylogenetic pattern analysis of eukaryotic genes
    Hulsen, Tim
    de Vlieg, Jacob
    Groenen, Peter M. A.
    [J]. BMC BIOINFORMATICS, 2006, 7 (1)