Determination of riboflavin in urine by capillary electrophoresis-blue light emitting diode-induced fluorescence detection combined with a stacking technique

A simple, inexpensive and, reliable method for the simultaneous, routine analysis of riboflavin in urine by capillary electrophoresis light emitting diode (LED)-induced fluorescence detection is described. Using a blue LED as the light source, the detection limit of riboflavin was determined to be 0.48 mug/ml and was improved to 20 ng/ml when a stacking technique was applied. In the analysis of an actual sample, various concentrations of riboflavin were distributed in the urine samples over a period, of 9 h after the ingestion of a vitamin B, tablet. (C) 2002 Elsevier Science B.V. All rights reserved.