Anthraquinone Glycoside Aloin Induces Osteogenic Initiation of MC3T3-E1 Cells: Involvement of MAPK Mediated Wnt and Bmp Signaling

被引：36
作者：
Pengjam, Yutthana [1 ,2 ]
Madhyastha, Harishkumar [1 ]
Madhyastha, Radha [1 ]
Yamaguchi, Yuya [1 ]
Nakajima, Yuichi [1 ]
Maruyama, Masugi [1 ]
机构：
[1] Miyazaki Univ, Fac Med, Dept Appl Physiol, Miyazaki 8891692, Japan
[2] Prince Songkla Univ, Fac Med Technol, Hat Yai 90110, Songkhla, Thailand
来源：
BIOMOLECULES & THERAPEUTICS | 2016年 / 24卷 / 02期
关键词：
Aloin; MC3T3-E1; cell; MAPK; Wnt; Bmp signaling; OSTEOBLAST DIFFERENTIATION; TRANSCRIPTION FACTOR; SKELETAL DEVELOPMENT; BONE; PATHWAYS; EXPRESSION; PROLIFERATION; MECHANISMS; APOPTOSIS; OSTERIX;
D O I：
10.4062/biomolther.2015.106
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Osteoporosis is a bone pathology leading to increased fracture risk and challenging the quality of life. The aim of this study was to evaluate the effect of an anthraquinone glycoside, aloin, on osteogenic induction of MC3T3-E1 cells. Aloin increased alkaline phosphatase (ALP) activity, an early differentiation marker of osteoblasts. Aloin also increased the ALP activity in adult human adipose-derived stem cells (hADSC), indicating that the action of aloin was not cell-type specific. Alizarin red S staining revealed a significant amount of calcium deposition in cells treated with aloin. Aloin enhanced the expression of osteoblast differentiation genes, Bmp-2, Runx2 and collagen 1a, in a dose-dependent manner. Western blot analysis revealed that noggin and inhibitors of p38 MAPK and SAPK/JNK signals attenuated aloin-promoted expressions of Bmp-2 and Runx2 proteins. siRNA mediated blocking of Wnt-5a signaling pathway also annulled the influence of aloin, indicating Wnt-5a dependent activity. Inhibition of the different signal pathways abrogated the influence of aloin on ALP activity, confirming that aloin induced MC3T3-E1 cells into osteoblasts through MAPK mediated Wnt and Bmp signaling pathway.
引用
收藏
页码：123 / 131
页数：9
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