A versatile multi-platform biochip surface attachment chemistry

被引：31

作者：

Manning, M ^{[1
]}

Harvey, S ^{[1
]}

Galvin, P ^{[1
]}

Redmond, G ^{[1
]}

机构：

[1] NMRC, Nanotechnol Grp, Cork, Ireland

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2003年 / 23卷 / 03期

关键词：

DNA; microarray; covalent attachment; probe immobilization; target hybridization;

D O I：

10.1016/S0928-4931(02)00285-0

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A versatile DNA spotting and immobilization method for covalent attachment of amino-modified probe oligonucleotides in microarray format at glass, native silicon dioxide and CVD silicon nitride substrates is reported. Optimal probe spot printing and attachment buffers are identified for each substrate. Relative areal densities of immobilized probes as measured by epi-fluorescence microscopy vary with substrate type reflecting differences in surface morphology and chemistry. Target oligonucleotide hybridization occurs at glass and nitride supported probe microarrays in an efficient and reproducible manner with excellent measured fluorescence signal-to-background. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：347 / 351

页数：5

共 4 条

[1] Robust and efficient synthetic method for forming DNA microarrays [J].