PER-1- and OXA-10-like β-lactamases in ceftazidime-resistant Pseudomonas aeruginosa isolates from intensive care unit patients in Istanbul, Turkey

被引:33
作者
Aktas, Z [1 ]
Poirel, L
Salcioglu, M
Özcan, PE
Midilli, K
Bal, Ç
Ang, Ö
Nordmann, P
机构
[1] Istanbul Univ, Istanbul Fac Med, Dept Microbiol & Clin Microbiol, Istanbul, Turkey
[2] Fac Med Paris Sud, Assistance Publ Hop Paris, Hop Bicetre, Serv Bacteriol Virol, Le Kremlin Bicetre, France
[3] Istanbul Univ, Istanbul Fac Med, Dept Anesthesiol, Istanbul, Turkey
[4] Istanbul Univ, Cerrahpasa Fac Med, Dept Microbiol & Clin Microbiol, Istanbul, Turkey
关键词
beta-lactamase; ceftazidime resistance; intensive care unit; OXA-10; PER-1; Pseudomonas aeruginosa;
D O I
10.1111/j.1469-0691.2004.01067.x
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The presence of PER-1- and OXA-10-like beta-lactamases was investigated by PCR in 49 ceftazidime-resistant Pseudomonas aeruginosa isolates from patients hospitalised in the 24-bed general intensive care unit of the Istanbul Faculty of Medicine during a 12-month period between February 1999 and February 2000. The clonal relatedness of the isolates was investigated by random amplified polymorphic DNA (RAPD) analysis, and the sequences of the PER-1 and OXA genes from all isolates were determined. The rates of resistance of the isolates to imipenem, aztreonam and cefepime were 98%, 92% and 96%, respectively, and to piperacillin and piperacillin-tazobactam were 41% and 37%, respectively. Using the double-disk synergy test, 37% (18/49) of the isolates were identified as extended-spectrum beta-lactamase producers. The PER-1 gene was identified in 86% (42/49) and the OXA-10 gene in 55% (27/49) of the ceftazidime-resistant isolates. Of isolates carrying the PER-1 gene, 48% (20/42) also carried the OXA-10 gene. The respective nucleotide sequences were identical for each isolate. Sixteen RAPD patterns were detected among the PER-1-positive isolates, but 60% (25/42) of the PER-1-positive isolates belonged to two distinct patterns, while the remainder exhibited a wide clonal diversity. The results indicated that the prevalence of PER-1- and OXA-10-like beta-lactamases remains high among ceftazidime-resistant P. aeruginosa isolates in Turkey.
引用
收藏
页码:193 / 198
页数:6
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