Evaluation of advanced platelet-rich fibrin (PRF) as a bio-carrier for ampicillin/sulbactam

被引:18
作者
Straub, Anton [1 ]
Vollmer, Andreas [1 ]
Lam, Thien-Tri [2 ]
Brands, Roman C. [1 ]
Stapf, Maximilian [3 ]
Scherf-Clavel, Oliver [3 ]
Bittrich, Max [4 ]
Fuchs, Andreas [1 ]
Kuebler, Alexander C. [1 ]
Hartmann, Stefan [1 ]
机构
[1] Univ Hosp Wurzburg, Dept Oral & Maxillofacial Plast Surg, Pleicherwall 2, D-97070 Wurzburg, Germany
[2] Univ Wurzburg, Inst Hyg & Microbiol, Josef Schneider St 2-E1, D-97080 Wurzburg, Germany
[3] Univ Wurzburg, Inst Pharm & Food Chem, D-97074 Wurzburg, Germany
[4] Univ Hosp Wurzburg, Dept Internal Med 2, Josef Schneider St 2, D-97080 Wurzburg, Germany
关键词
Osteonecrosis of the jaw; Osteoradionecrosis; Antiresorptive drug-related osteonecrosis of the jaw; ARONJ; Oral microbiome; Agar diffusion test; BISPHOSPHONATE-RELATED OSTEONECROSIS; MEDICATION-RELATED OSTEONECROSIS; JAW; PLASMA;
D O I
10.1007/s00784-022-04663-y
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objectives Mechanisms of wound healing are often impaired in patients with osteonecrosis of the jaw (ONJ). According to the guidelines for the treatment of this disease, early surgical intervention is indicated. However, surgery often faces complications such as wound healing disorders. The application of platelet-rich fibrin (PRF) after necrosectomy between bone and mucosa may constitute a promising approach to improve surgical results. An aspect that was not investigated until now is that PRF acts as a "bio-carrier" for antibiotics previously applied intravenously. Materials and methods We investigated the antimicrobial properties of PRF in 24 patients presenting ONJ undergoing systemic antibiosis with ampicillin/sulbactam. We measured the concentration of ampicillin/sulbactam in plasma and PRF and performed agar diffusion tests. Ampicillin/sulbactam was applied intravenously to the patient 10 minutes for blood sampling for PRF. No further incorporation of patients' blood or PRF product with antibiotic drugs was obtained. Four healthy patients served as controls. Results Our results revealed that PRF is highly enriched with ampicillin/sulbactam that is released to the environment. The antibiotic concentration in PRF was comparable to the plasma concentration of ampicillin/sulbactam. The inhibition zone (IZ) of PRF was comparable to the standard ampicillin/sulbactam discs used in sensitivity testing. Conclusions The results of our study demonstrated that PRF is a reliable bio-carrier for systemic applied antibiotics and exhibits a large antimicrobial effect.
引用
收藏
页码:7033 / 7044
页数:12
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