Short-Term Low Temperature Induces Nitro-Oxidative Stress that Deregulates the NADP-Malic Enzyme Function by Tyrosine Nitration in Arabidopsis thaliana

被引：18

作者：

Begara-Morales, Juan C. ^{[1
]}

Sanchez-Calvo, Beatriz ^{[1
]}

Gomez-Rodriguez, Maria, V ^{[1
]}

Chaki, Mounira ^{[1
]}

Valderrama, Raquel ^{[1
]}

Mata-Perez, Capilla ^{[1
]}

Lopez-Jaramillo, Javier ^{[2
]}

Corpas, Francisco J. ^{[3
]}

Barroso, Juan B. ^{[1
]}

机构：

[1] Univ Jaen, Grp Biochem & Cell Signaling Nitr Oxide, Ctr Adv Studies Olive Grove & Olive Oils, Fac Expt Sci,Dept Expt Biol, Campus Las Lagunillas S-N, E-23071 Jaen, Spain

[2] Univ Granada, Fac Sci, Inst Biotechnol, Dept Organ Chem, E-18071 Granada, Spain

[3] CSIC, Grp Antioxidants Free Rad & Nitr Oxide Biotechnol, Dept Biochem Cell & Mol Biol Plants, Estn Expt Zaidin, C Prof Albareda 1, E-18080 Granada, Spain

来源：

ANTIOXIDANTS | 2019年 / 8卷 / 10期

关键词：

NADP malic enzyme; low temperature; nitric oxide; tyrosine nitration; peroxynitrite; reactive oxygen species; reactive nitrogen species; nitro-oxidative stress; DEPENDENT THIOREDOXIN REDUCTASE; COLD-ACCLIMATION; FREEZING TOLERANCE; GENE-EXPRESSION; ISOCITRATE DEHYDROGENASE; DIFFERENTIAL REGULATION; GLUTATHIONE-REDUCTASE; TRANSCRIPTION FACTORS; NITROSATIVE STRESS; OXIDE SYNTHASE;

D O I：

10.3390/antiox8100448

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Low temperature (LT) negatively affects plant growth and development via the alteration of the metabolism of reactive oxygen and nitrogen species (ROS and RNS). Among RNS, tyrosine nitration, the addition of an NO2 group to a tyrosine residue, can modulate reduced nicotinamide-dinucleotide phosphate (NADPH)-generating systems and, therefore, can alter the levels of NADPH, a key cofactor in cellular redox homeostasis. NADPH also acts as an indispensable electron donor within a wide range of enzymatic reactions, biosynthetic pathways, and detoxification processes, which could affect plant viability. To extend our knowledge about the regulation of this key cofactor by this nitric oxide (NO)-related post-translational modification, we analyzed the effect of tyrosine nitration on another NADPH-generating enzyme, the NADP-malic enzyme (NADP-ME), under LT stress. In Arabidopsis thaliana seedlings exposed to short-term LT (4 degrees C for 48 h), a 50% growth reduction accompanied by an increase in the content of superoxide, nitric oxide, and peroxynitrite, in addition to diminished cytosolic NADP-ME activity, were found. In vitro assays confirmed that peroxynitrite inhibits cytosolic NADP-ME2 activity due to tyrosine nitration. The mass spectrometric analysis of nitrated NADP-ME2 enabled us to determine that Tyr-73 was exclusively nitrated to 3-nitrotyrosine by peroxynitrite. The in silico analysis of the Arabidopsis NADP-ME2 protein sequence suggests that Tyr73 nitration could disrupt the interactions between the specific amino acids responsible for protein structure stability. In conclusion, the present data show that short-term LT stress affects the metabolism of ROS and RNS, which appears to negatively modulate the activity of cytosolic NADP-ME through the tyrosine nitration process.

引用

页数：20