Measuring and modeling the influence of salinity change on the transport behaviour of Escherichia coli through quartz sand

Pathogenic bacteria can be discharged in the environment through natural as well as anthropogenic activities. Once in the environment, they may contaminate soil and sediments and migrate towards water bodies. Transient chemical conditions may occur in soil/sediments and favor mobilization of bacteria, e.g., upon the reduction of salinity (or ionic strength). However, the magnitude of this phenomenon and its relationship with particle size is not well understood, yet. In this work, we investigated the transport of Escherichia coli under variable salinity conditions (between 1 and 20 part per thousand, ppt) and for different soil grain sizes (between 150 and 710 mu m). A model developed in our group was applied in this work. It couples bacteria and salinity transport equations in order to account for transient water composition in the description of bacteria migration. The model was calibrated and validated with laboratory experiments. The tests were monitored continuously with UV-Vis spectroscopy, which allowed to record highly resolved concentration fronts. The results show that salinity increases the retardation of the bacteria. Upon salinity drop, a release of bacteria occurs forming a peak whose magnitude increases with salinity change. This effect becomes more important as the grain size decreases. Simulations suggest that the dominant retention mechanism is attachment for coarse sand and straining for fine sand. The retention can be reversed as the salinity is reduced causing a sudden bacteria mobilization. Such a behaviour may have important implications on microbial contamination of water bodies when soil/sediments undergo transient chemical conditions.