Development and evaluation of a sensitive recombinase aided amplification assay for rapid detection of Vibrio parahaemolyticus

被引：13

作者：

Feng, Zhi-shan ^{[1
,2
]}

Li, Jing-yi ^{[1
,2
,3
]}

Zhang, Jing-yun ^{[4
]}

Li, Feng-yu ^{[1
,2
,3
]}

Guan, Hong-xia ^{[5
]}

Zhang, Rui-qing ^{[3
]}

Liu, Hong ^{[6
]}

Guo, Qi ^{[7
]}

Shen, Xin-xin ^{[3
]}

Kan, Biao ^{[4
]}

Ma, Xue-jun ^{[3
]}

机构：

[1] Hebei Med Univ, Shijiazhuang 050031, Hebei, Peoples R China

[2] Hebei Gen Hosp, Shijiazhuang 050070, Hebei, Peoples R China

[3] Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, NHC Key Lab Med Virol & Viral Dis, Beijing 102206, Peoples R China

[4] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable DiseaseControl & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China

[5] Wuxi Ctr Dis Control & Prevent, Wuxi 214023, Jiangsu, Peoples R China

[6] Shandong Univ Technol, Shandong Prov Res Ctr Bioinformat Engn & Tech, Sch Life Sci & Med, Zibo 255049, Shandong, Peoples R China

[7] Capital Inst Pediat, Lab Virol, Beijing Key Lab OfEtiol Viral Dis Children, Beijing 100020, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2022年 / 193卷

关键词：

Rapid detection; Recombinase aided amplification; Vibrio parahaemolyticus; ToxR; Sensitivity; Specificity; SALMONELLA;

D O I：

10.1016/j.mimet.2021.106404

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Vibrio parahaemolyticus (V. parahaemolyticus) is a widely distributed pathogen in the coastal areas, which causes food poisoning and leads to gastroenteritis and sepsis. Therefore, developing a simple, sensitive, and rapid detection method for V. parahaemolyticus is a major concern globally. This study established a sensitive and rapid technique based on recombinase aided amplification (RAA) to detect V. parahaemolyticus. The RAA reaction was carried out successfully at 39 degrees C within 30 min. The sensitivity of the RAA assay was 101 copies/mu L using the recombinant plasmid and 10-3 ng/mu L using the V. parahaemolyticus strain. In addition, RAA directly detected 7 x 103 CFU/mL of simulated fecal samples and 0.1 CFU/mL after enrichment for 4 h. The sensitivity and specificity of the RAA assay using fecal and fish samples were 100% similar to that of the real-time PCR. We conclude that the RAA assay is an ideal screening method for detecting V. parahaemolyticus due to its rapidity, high accuracy, and simplicity in operation.

引用

页数：7

共 36 条

[1] Characterizing the Adherence Profiles of Virulent Vibrio parahaemolyticus Isolates [J].

Aagesen, Alisha M. ;

Phuvasate, Sureerat ;

Su, Yi-Cheng ;

Hase, Claudia C. .

MICROBIAL ECOLOGY, 2018, 75 (01) :152-162

[2] Evaluation of loop-mediated isothermal amplification assay along with conventional and real-time PCR assay for sensitive detection of pathogenic Vibrio parahaemolyticus from seafood sample without enrichment [J].

Anupama, Karanth Padyana ;

Nayak, Ashwath ;

Karunasagar, Iddya ;

Karunasagar, Indrani ;

Maiti, Biswajit .

MOLECULAR BIOLOGY REPORTS, 2021, 48 (01) :1009-1016

[3] Rapid visual detection of Vibrio parahaemolyticus in seafood samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye [J].

Anupama, Karanth Padyana ;

Nayak, Ashwath ;

Karunasagar, Indrani ;

Maiti, Biswajit .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2020, 36 (05)

[4] Non-Cholera Vibrios: The Microbial Barometer of Climate Change [J].

Baker-Austin, Craig ;

Trinanes, Joaquin ;

Gonzalez-Escalona, Narjol ;

Martinez-Urtaza, Jaime .

TRENDS IN MICROBIOLOGY, 2017, 25 (01) :76-84

[5] Vibrio species involved in seafood-borne outbreaks (Vibrio cholerae, V-parahaemolyticus and V-vulnificus): Review of microbiological versus recent molecular detection methods in seafood products [J].

Bonnin-Jusserand, Maryse ;

Copin, Stephanie ;

Le Bris, Cedric ;

Brauge, Thomas ;

Gay, Melanie ;

Brisabois, Anne ;

Grard, Thierry ;

Midelet-Bourdin, Graziella .

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 2019, 59 (04) :597-610

[6] Distribution and dynamics of epidemic and pandemic Vibrio parahaemolyticus virulence factors [J].

Ceccarelli, Daniela ;

Hasan, Nur A. ;

Huq, Anwar ;

Colwell, Rita R. .

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2013, 3

[7] Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay Combined with Enrichment Culture for Rapid Detection of Very Low Numbers of Vibrio parahaemolyticus in Seafood Samples [J].

Di, Huiling ;

Ye, Lei ;

Neogi, Sucharit Basu ;

Meng, Hecheng ;

Yan, He ;

Yamasaki, Shinji ;

Shi, Lei .

BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2015, 38 (01) :82-87

[8] Antibiotic resistance of Vibrio parahaemolyticus and Vibrio vulnificus in various countries: A review [J].

Elmahdi, Sara ;

DaSilva, Ligia V. ;

Parveen, Salina .

FOOD MICROBIOLOGY, 2016, 57 :128-134

[9] Development and evaluation of a rapid and sensitive RPA assay for specific detection of Vibrio parahaemolyticus in seafood [J].

Geng, Yunyun ;

Tan, Ke ;

Liu, Libing ;

Sun, Xiao Xia ;

Zhao, Baohua ;

Wang, Jianchang .

BMC MICROBIOLOGY, 2019, 19 (01)

[10] Potentially human pathogenic Vibrio spp. in a coastal transect: Occurrence and multiple virulence factors [J].

Hackbusch, Sidika ;

Wichels, Antje ;

Gimenez, Luis ;

Doepke, Hilke ;

Gerdts, Gunnar .

SCIENCE OF THE TOTAL ENVIRONMENT, 2020, 707

← 1 2 3 4 →